Indik Stanislav, Valícek Lubomír, Smíd Bedrich, Dvoráková Hana, Rodák Ladislav
Research Institute of Virology and Biomedicine, University of Veterinary Medicine, Veterinärplatz 1, Vienna, Austria.
Vet Microbiol. 2006 Oct 31;117(2-4):276-83. doi: 10.1016/j.vetmic.2006.06.020. Epub 2006 Aug 1.
Astroviral infection has been described as one of the causes of porcine diarrhoeal disease. Here we describe the detection of astrovirus-like particles by electron microscopy in a diarrhoeal specimen. Furthermore, a cytopathic virus was isolated and propagated in an established porcine kidney cell line, PK-15. Reverse transcription and PCR performed with astrovirus-specific primers amplified a product with the expected size. Sequencing of the PCR product revealed that the virus observed by electron microscopy and propagated in the porcine cell line is an astrovirus, showing 86% identity at the nucleotide level with the only known porcine astrovirus, PAstV. Phylogenetic analysis clustered the novel isolate, Sb4685, together with PAstV in a broad clade comprising mammalian astroviruses.
星状病毒感染被认为是猪腹泻病的病因之一。在此，我们描述了通过电子显微镜在一份腹泻标本中检测到类星状病毒颗粒。此外，从腹泻标本中分离出一种细胞病变病毒，并在已建立的猪肾细胞系PK - 15中进行培养。用星状病毒特异性引物进行逆转录和PCR扩增出预期大小的产物。PCR产物测序显示，通过电子显微镜观察到并在猪细胞系中培养的病毒是一种星状病毒，在核苷酸水平上与唯一已知的猪星状病毒PAstV具有86%的同一性。系统发育分析将新分离株Sb4685与PAstV聚集在一个包含哺乳动物星状病毒的广泛进化枝中。
