Marco Jorge D, Uezato Hiroshi, Mimori Tatsuyuki, Barroso Paola A, Korenaga Masataka, Nonaka Shigeo, Basombrío Miguel A, Taranto Néstor J, Hashiguchi Yoshihisa
Department of Parasitology, Kochi Medical School, Kochi University, Kohasu, Kochi, Japan.
Am J Trop Med Hyg. 2006 Aug;75(2):256-60.
Recently, two techniques, polymerase chain reaction (PCR) amplification and sequencing of cytochrome b gene (cyt b gene sequencing) and polymorphism-specific PCR (PS-PCR) were recommended for Leishmania species identification. Before this study, however, the accuracy of these methods had not been tested against the multilocus enzyme electrophoresis, the current gold standard technique on this task. Therefore, a trial was done for the first time to compare the results obtained by these techniques, using 17 Argentinean Leishmania stocks in independent assays. For all the stocks examined, the same results at species level were obtained by the three techniques. Among them, 14 were assigned to L. (Viannia) braziliensis, and three to L. (V.) guyanensis. The two techniques, cyt b gene sequencing and PS-PCR, were able to distinguish between all the proven species responsible for leishmaniases in Argentina. Thus, both techniques were validated and could be used independently for the species designation of Leishmania parasites in the country.
最近，推荐了两种技术用于利什曼原虫物种鉴定，即聚合酶链反应（PCR）扩增和细胞色素b基因测序（cyt b基因测序）以及多态性特异性PCR（PS-PCR）。然而，在本研究之前，这些方法的准确性尚未与多位点酶电泳（目前这项任务的金标准技术）进行过对比测试。因此，首次进行了一项试验，在独立检测中使用17株阿根廷利什曼原虫菌株来比较这些技术所获得的结果。对于所有检测的菌株，这三种技术在物种水平上获得了相同的结果。其中，14株被鉴定为巴西利什曼原虫（Viannia亚属），3株为圭亚那利什曼原虫（V.亚属）。细胞色素b基因测序和PS-PCR这两种技术能够区分阿根廷所有已证实的引起利什曼病的物种。因此，这两种技术均得到验证，可独立用于该国利什曼原虫寄生虫的物种鉴定。
