Ray Pratima, Fenaux Martijn, Sharma Sumit, Malik Jyoti, Subodh Swati, Bhatnagar Shinjini, Greenberg Harry, Glass Roger I, Gentsch Jon, Bhan M K
Department of Pediatrics, Center for Diarrheal Disease Research, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India.
J Infect Dis. 2006 Sep 1;194(5):588-93. doi: 10.1086/505878. Epub 2006 Jul 26.
Rotaviral antigen and RNA have recently been identified in the serum of patients with rotaviral gastroenteritis, but the roles they play in disease remains undetermined.
Rotaviral antigen and RNA were quantified by enzyme-linked immunosorbant assay and by quantitative reverse-transcription polymerase chain reaction in stool and serum specimens from children with rotaviral diarrhea (n=102), children with nonrotaviral diarrhea (n=40), and nondiarrheal control children (n=30).
Rotaviral antigenemia was detected in 64%, 3%, and 0% of the children with rotaviral diarrhea, the children with nonrotaviral diarrhea, and the nondiarrheal control children, respectively. The level of rotaviral antigen in serum was approximately 2x10(2) -fold lower than that in stool, and a moderate correlation was observed between the 2 levels. Rotaviral RNA was detected in 93% of the antigen-positive serum specimens. The median number of RNA copies in serum was approximately 1 x 10(5) -fold lower than that in stool, and no correlation was observed between the 2 levels. Serum levels of both antigen and RNA were inversely associated with baseline titers of rotaviral serum immunoglobulin G (P<.01). Antigenemia was also associated with G1 serotype.
Rotaviral antigenemia and viremia were common in children with rotaviral diarrhea, but antigen and RNA levels in serum were substantially lower than those in stool. Antigenemia was associated with infection with G1 strains and with low baseline titers of rotaviral serum antibody.
最近在轮状病毒性胃肠炎患者的血清中发现了轮状病毒抗原和RNA，但它们在疾病中所起的作用仍未确定。
采用酶联免疫吸附测定法和定量逆转录聚合酶链反应，对轮状病毒腹泻患儿（n = 102）、非轮状病毒腹泻患儿（n = 40）和无腹泻对照儿童（n = 30）的粪便和血清标本中的轮状病毒抗原和RNA进行定量。
轮状病毒腹泻患儿、非轮状病毒腹泻患儿和无腹泻对照儿童中，分别有64%、3%和0%检测到轮状病毒血症。血清中轮状病毒抗原水平比粪便中低约2×10²倍，且两者水平之间存在中度相关性。在93%的抗原阳性血清标本中检测到轮状病毒RNA。血清中RNA拷贝数的中位数比粪便中低约1×10⁵倍，且两者水平之间未观察到相关性。抗原和RNA的血清水平均与轮状病毒血清免疫球蛋白G的基线滴度呈负相关（P<0.01）。病毒血症也与G1血清型有关。
轮状病毒血症和病毒血症在轮状病毒腹泻患儿中很常见，但血清中的抗原和RNA水平明显低于粪便中的水平。病毒血症与G1毒株感染以及轮状病毒血清抗体的低基线滴度有关。
