Collen T, Doel T R
Vaccine Research Department, AFRC Institute for Animal Health, Pirbright Laboratory, Surrey, U.K.
J Gen Virol. 1990 Feb;71 ( Pt 2):309-15. doi: 10.1099/0022-1317-71-2-309.
Lymphoproliferation against foot-and-mouth disease (FMD) virus was examined using peripheral blood mononuclear cells from vaccinated cattle. Ten weeks after revaccination the optimum conditions for proliferation were obtained with 1 microgram/ml of purified virus after 5 to 6 days in culture. This contrasted with the response at 20 months post-revaccination, when the response required less antigen and showed a peak response after 3 to 4 days in culture. Proliferation was specific for FMD virus, but was cross-reactive between serotypically distinct strains of the virus. The proliferative response to isolated virus proteins (VP) involved all three major capsid proteins (VP1, -2 and -3), although the proliferation of lymphocytes from heterotypically vaccinated cattle was due to VP3. Furthermore, the response induced by purified virus, chemically fixed virus and subunit virus particles was indistinguishable and thus it is likely that processing was required for the induction of proliferation. Together these data strongly suggest that FMD virus-induced lymphoproliferation is T cell-mediated and that VP3 may contain dominant, cross-reactive sequences.
利用接种疫苗牛的外周血单个核细胞检测了针对口蹄疫（FMD）病毒的淋巴细胞增殖情况。再次接种疫苗10周后，在培养5至6天后，用1微克/毫升纯化病毒获得了增殖的最佳条件。这与再次接种疫苗20个月后的反应形成对比，那时反应所需抗原较少，且在培养3至4天后出现反应峰值。增殖是口蹄疫病毒特异性的，但在病毒血清型不同的毒株之间存在交叉反应。对分离的病毒蛋白（VP）的增殖反应涉及所有三种主要衣壳蛋白（VP1、-2和-3），尽管来自异型接种牛的淋巴细胞增殖是由VP3引起的。此外，纯化病毒、化学固定病毒和亚单位病毒颗粒诱导的反应无法区分，因此诱导增殖可能需要进行加工处理。这些数据共同有力地表明，口蹄疫病毒诱导的淋巴细胞增殖是由T细胞介导的，并且VP3可能包含显性的、交叉反应性序列。
