Nougayrède Jean-Philippe, Homburg Stefan, Taieb Frédéric, Boury Michèle, Brzuszkiewicz Elzbieta, Gottschalk Gerhard, Buchrieser Carmen, Hacker Jörg, Dobrindt Ulrich, Oswald Eric
INRA, UMR1225, Ecole Nationale Vétérinaire de Toulouse, Toulouse F-31076, France.
Science. 2006 Aug 11;313(5788):848-51. doi: 10.1126/science.1127059.
Transient infection of eukaryotic cells with commensal and extraintestinal pathogenic Escherichia coli of phylogenetic group B2 blocks mitosis and induces megalocytosis. This trait is linked to a widely spread genomic island that encodes giant modular nonribosomal peptide and polyketide synthases. Contact with E. coli expressing this gene cluster causes DNA double-strand breaks and activation of the DNA damage checkpoint pathway, leading to cell cycle arrest and eventually to cell death. Discovery of hybrid peptide-polyketide genotoxins in E. coli will change our view on pathogenesis and commensalism and open new biotechnological applications.
系统发育B2组的共生和肠道外致病性大肠杆菌对真核细胞的短暂感染会阻断有丝分裂并诱导巨细胞形成。这一特性与一个广泛传播的基因组岛有关，该基因组岛编码巨大的模块化非核糖体肽和聚酮合酶。与表达该基因簇的大肠杆菌接触会导致DNA双链断裂并激活DNA损伤检查点途径，导致细胞周期停滞并最终导致细胞死亡。在大肠杆菌中发现杂合肽-聚酮基因毒素将改变我们对发病机制和共生关系的看法，并开启新的生物技术应用。
