Consales C A, Mendonca R Z, Gallina N M, Pereira C A
Laboratorio de Imunologia Viral, Instituto Butantan, São Paulo, Brazil.
J Virol Methods. 1990 Mar;27(3):277-85. doi: 10.1016/0166-0934(90)90096-x.
Both fixed and street rabies virus when cultivated in McCoy cells caused cytopathic changes 24 to 72 h after infection, depending on the multiplicity of infection. The cytopathic effect (CPE) was easily recognizable and resembles that induced by other members of the Rhabdovirus group, such as vesicular stomatitis virus, in several cell cultures. Higher titers of the Pasteur strain (PV) of fixed rabies virus were found in supernatants of McCoy cells when compared to those in VERO cells. The virus titer increased with the number of passages attaining a high titer after three passages. Rabies antigens were detected by direct immunofluorescence labeling in most McCoy cells of the infected culture, and specific antibodies neutralized the virus growth and CPE. There was also inhibition by treatment of the cells with human interferon (HuIFN) -alpha or -gamma, but not by murine interferon (MuIFN) -alpha, -beta or -gamma. Rabies-infected McCoy cell cultures may provide a useful assay system, based on the induction of CPE, the high virus production and the sensitivity to IFN.
固定毒株和街毒株狂犬病病毒在 McCoy 细胞中培养时，感染后 24 至 72 小时会引起细胞病变效应，具体时间取决于感染复数。细胞病变效应（CPE）很容易识别，在几种细胞培养物中类似于由弹状病毒科的其他成员（如水泡性口炎病毒）诱导的效应。与 VERO 细胞相比，在 McCoy 细胞的上清液中发现固定狂犬病病毒巴斯德毒株（PV）的滴度更高。病毒滴度随着传代次数的增加而升高，传代三次后达到高滴度。通过直接免疫荧光标记在感染培养物的大多数 McCoy 细胞中检测到狂犬病抗原，并且特异性抗体中和了病毒生长和 CPE。用人干扰素（HuIFN）-α 或 -γ 处理细胞也有抑制作用，但用鼠干扰素（MuIFN）-α、-β 或 -γ 处理则没有。基于 CPE 的诱导、高病毒产量和对干扰素的敏感性，感染狂犬病的 McCoy 细胞培养物可能提供一个有用的检测系统。
