资源有限实验室的抗真菌药敏试验方法

Antifungal susceptibility testing method for resource constrained laboratories.

作者信息

Khan S, Singhal S, Mathur T, Upadhyay D J, Rattan A

机构信息

Department of Infectious Diseases, Ranbaxy Research Laboratories, Gurgaon - 122 001, Haryana, India.

出版信息

Indian J Med Microbiol. 2006 Jul;24(3):171-6.

PMID:16912435

Abstract

PURPOSE

In resource-constrained laboratories of developing countries determination of antifungal susceptibility testing by NCCLS/CLSI method is not always feasible. We describe herein a simple yet comparable method for antifungal susceptibility testing.

METHODS

Reference MICs of 72 fungal isolates including two quality control strains were determined by NCCLS/CLSI methods against fluconazole, itraconazole, voriconazole, amphotericin B and cancidas. Dermatophytes were also tested against terbinafine. Subsequently, on selection of optimum conditions, MIC was determined for all the fungal isolates by semisolid antifungal agar susceptibility method in Brain heart infusion broth supplemented with 0.5% agar (BHIA) without oil overlay and results were compared with those obtained by reference NCCLS/CLSI methods.

RESULTS

Comparable results were obtained by NCCLS/CLSI and semisolid agar susceptibility (SAAS) methods against quality control strains. MICs for 72 isolates did not differ by more than one dilution for all drugs by SAAS.

CONCLUSIONS

SAAS using BHIA without oil overlay provides a simple and reproducible method for obtaining MICs against yeast, filamentous fungi and dermatophytes in resource-constrained laboratories.

摘要

目的

在发展中国家资源有限的实验室中，采用美国国家临床实验室标准化委员会（NCCLS）/临床和实验室标准协会（CLSI）方法进行抗真菌药敏试验并不总是可行的。我们在此描述一种简单但具有可比性的抗真菌药敏试验方法。

方法

采用NCCLS/CLSI方法测定包括两种质控菌株在内的72株真菌分离株对氟康唑、伊曲康唑、伏立康唑、两性霉素B和卡泊芬净的参考最低抑菌浓度（MIC）。皮肤癣菌还进行了对特比萘芬的药敏试验。随后，在选择最佳条件后，采用半固体抗真菌琼脂药敏方法在补充有0.5%琼脂的脑心浸液肉汤（BHIA）中测定所有真菌分离株的MIC，不覆盖油层，并将结果与通过参考NCCLS/CLSI方法获得的结果进行比较。