Cheng Ke-Ke, Zhang Jian-An, Liu De-Hua, Sun Yan, Yang Ming-De, Xu Jing-Ming
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, P.R. China.
Biotechnol Lett. 2006 Nov;28(22):1817-21. doi: 10.1007/s10529-006-9158-8. Epub 2006 Aug 16.
Broth containing 152 g glycerol l(-1) from Candida krusei culture was converted to 1,3-propanediol by Klebsiella pneumoniae. Residual glucose in the broth promoted growth of K. pneumoniae while acetate was inhibitory. After desalination treatment of glycerol broth by electrodialysis, the acetate in the broth was removed. A fed-batch culture with electrodialytically pretreated broth as substrate was developed giving 53 g 1,3-propanediol l(-1) with a yield of 0.41 g g(-1) glycerol and a productivity of 0.94 g l(-1) h(-1).
来自克鲁斯假丝酵母培养物的含152 g甘油l⁻¹的肉汤被肺炎克雷伯菌转化为1,3 - 丙二醇。肉汤中的残留葡萄糖促进了肺炎克雷伯菌的生长，而乙酸盐具有抑制作用。通过电渗析对甘油肉汤进行脱盐处理后，肉汤中的乙酸盐被去除。开发了一种以经电渗析预处理的肉汤为底物的分批补料培养方法，得到了53 g 1,3 - 丙二醇l⁻¹，甘油产率为0.41 g g⁻¹，生产效率为0.94 g l⁻¹ h⁻¹。
