Jang Myoseon, Ghio Andrew J, Cao Gang
Department of Environmental Sciences and Engineering, CB# 7431, Rosenau Hall, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
Chem Res Toxicol. 2006 Aug;19(8):1044-50. doi: 10.1021/tx0503597.
Toxicological investigation suggests that exposures to complex secondary organic aerosol (SOA) products can result in adverse health effects in biological systems. However, the mechanism of adverse health effects is not yet understood. One of the major restrictions in studies of health effects of SOA is a particle exposure technique. In this study, we applied an innovative soft targeting technology using magnetic nanoparticles (MNP) to deliver SOAs onto target biological systems under a magnetic field. The exploratory exposure technology using MNP was demonstrated for the SOAs created from the reaction of ozone with alpha-pinene in an indoor Teflon film chamber. SOA increased the release of the proinflammatory mediator interleukin-8 by respiratory epithelial cells. These results support that MNP can effectively deliver SOAs to epithelial cells in vitro resulting in a significant biological effects.
毒理学调查表明，接触复杂的二次有机气溶胶（SOA）产物可导致生物系统出现不良健康影响。然而，不良健康影响的机制尚不清楚。SOA健康影响研究的主要限制之一是颗粒暴露技术。在本研究中，我们应用了一种创新的软靶向技术，利用磁性纳米颗粒（MNP）在磁场作用下将SOA传递到目标生物系统上。在室内聚四氟乙烯薄膜腔室中，对由臭氧与α-蒎烯反应产生的SOA，展示了使用MNP的探索性暴露技术。SOA增加了呼吸道上皮细胞促炎介质白细胞介素-8的释放。这些结果支持MNP可以在体外有效地将SOA传递到上皮细胞，从而产生显著的生物学效应。
