Clinical Research Branch, Human Studies Division, National Health and Environmental Effects Research Laboratory, Environmental Protection Agency, Research Triangle Park, North Carolina, USA.
Inhal Toxicol. 2009 Jul;21(8):659-67. doi: 10.1080/08958370802406282.
Numerous industrial applications for man-made nanoparticles have been proposed. Interactions of nanoparticles with agents in the atmosphere may impact human health. We tested the postulate that in vitro exposures of respiratory epithelial cells to airborne magnetic nanoparticles (MNP; Fe(3)O(4)) with and without a secondary organic aerosol (SOA) and an inorganic acid could affect iron homeostasis, oxidative stress, and interleukin (IL)-8 release. Cell iron concentrations were increased after exposures to MNP and values were further elevated with co-exposures to either SOA or inorganic acid. Increased expression of ferritin and elevated levels of RNA for DMT1, proteins for iron storage and transport respectively, followed MNP exposures, but values were significant for only those with co-exposures to inorganic acid and organic aerosols. Cell iron concentration corresponded to a measure of oxidative stress in the airway epithelial cells; MNP with co-exposures to SOA and inorganic acid increased both available metal and indices of oxidant generation. Finally, the release of a proinflammatory cytokine (i.e. IL-8) by the exposed cells similarly increased with cell iron concentration. We conclude that MNP can interact with a SOA and an inorganic acid to present metal in a catalytically reactive state to cultured respiratory cells. This produces an oxidative stress to affect a release of IL-8.
已经提出了许多人造纳米粒子的工业应用。纳米粒子与大气中的物质相互作用可能会影响人类健康。我们验证了以下假设，即在体外将呼吸上皮细胞暴露于空气中的磁性纳米粒子（MNP；Fe(3)O(4)）及其与二次有机气溶胶（SOA）和无机酸的共暴露，可能会影响铁稳态、氧化应激和白细胞介素（IL）-8 的释放。暴露于 MNP 后细胞内铁浓度增加，与 SOA 或无机酸共暴露后进一步升高。MNP 暴露后 ferritin 的表达增加，DMT1 的 RNA 水平升高，分别代表铁的储存和运输蛋白，仅在与无机酸和有机气溶胶共暴露时才有统计学意义。细胞内铁浓度与气道上皮细胞内氧化应激的一个衡量标准相对应；与 SOA 和无机酸共暴露的 MNP 增加了可利用金属和氧化剂生成的指标。最后，暴露细胞中促炎细胞因子（即 IL-8）的释放也随着细胞内铁浓度的增加而增加。我们得出结论，MNP 可以与 SOA 和无机酸相互作用，将金属呈现在具有催化反应性的状态，从而对培养的呼吸细胞产生氧化应激，进而影响 IL-8 的释放。
