Kooshki Fahime, Tabatabaei Fahimeh S, Tajik Sahar, Aayan Azadeh
Department of Pediatric Dentistry, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Dent Res J (Isfahan). 2018 Jul-Aug;15(4):289-294.
Nowadays, health-care companies use different antimicrobial agents in toothpastes to reduce oral microorganisms. The aim of this study was to investigate the antimicrobial effects of one Iranian herbal toothpaste in different concentrations compared with the chemical type on oral microorganisms .
In this experimental study, the antimicrobial effect of one Iranian herbal toothpaste in comparison with its chemical type at three concentrations of 1, 1:1, and 1:3 on (SM), (LB), and (CA), respectively, were studied by agar disc diffusion method. The microorganisms were cultured on 21 plates. Then, four sterile paper discs were placed on each plate and the extracts were placed on them in prepared concentrations and incubated at 37°C ± 0°C for 24 h. The diameter of the inhibition zone around the discs was then measured in millimeters and recorded two-way ANOVA, one-way ANOVA tests, and regarding the difference variances, Tamhane supplementary tests were used at the significance level of < 0.05.
According to the results of this study, the full concentration of Iranian herbal toothpaste on SM, LB, and CA microorganisms had higher antimicrobial effect than the other two concentrations. This difference was statistically significant ( < 0.05). Furthermore, all the three toothpastes at full concentration had the same antimicrobial activity ( < 0.05). The antimicrobial effect of herbal toothpaste decreased significantly compared with the chemical toothpaste while the concentration decreased ( < 0.05).
At full concentration, herbal and chemical toothpastes have the same antimicrobial effect, but by reducing the concentration, the antimicrobial effect of herbal toothpaste is reduced compared with the chemical one.
如今，医疗保健公司在牙膏中使用不同的抗菌剂来减少口腔微生物。本研究的目的是调查一种伊朗草药牙膏在不同浓度下与化学型牙膏相比对口腔微生物的抗菌效果。
在本实验研究中，通过琼脂纸片扩散法研究了一种伊朗草药牙膏与其化学型牙膏在1、1:1和1:3三种浓度下分别对变形链球菌（SM）、嗜酸乳杆菌（LB）和血链球菌（CA）的抗菌效果。将微生物接种在21个平板上。然后，在每个平板上放置四个无菌纸片，并将提取物按制备好的浓度放置在纸片上，于37°C±0°C孵育24小时。然后用毫米测量纸片周围抑菌圈的直径并记录。采用双向方差分析、单向方差分析测试，对于差异方差，在显著性水平P<0.05时使用塔姆黑尼补充检验。
根据本研究结果，伊朗草药牙膏全浓度对SM、LB和CA微生物的抗菌效果高于其他两种浓度。这种差异具有统计学意义（P<0.05）。此外，所有三种全浓度牙膏具有相同的抗菌活性（P<0.05）。与化学牙膏相比，草药牙膏浓度降低时抗菌效果显著降低（P<0.05）。
全浓度时，草药牙膏和化学牙膏具有相同的抗菌效果，但随着浓度降低，草药牙膏的抗菌效果相比化学牙膏有所降低。
