Prasanth Manupati
Assistant Professor, School of Microbiology, Mahatma Gandhi National Institute of Research and Social Action (MGNIRSA), Hyderabad, Andhra Pradesh, India.
Dent Res J (Isfahan). 2011 Spring;8(2):85-94.
Anti-microbial agents have been used as a chemotherapeutic agent to improve oral health. This in vitro study was carried out to determine antimicrobial efficacy of different toothpastes and mouthrinses against the oral pathogens.
A total of five toothpastes and five mouthrinses were tested for their antimicrobial activity against three oral pathogens namely, Streptococcus mutans (MTCC 890), Escherichia coli (MTCC 579) and Candida albicans (MTCC 854) by well agar diffusion assay. Statistical Analysis was performed using a statistical package, SPSS windows version 15, by applying mean values using analysis of variance (ANOVA) with post-hoc least square differences (LSD) method(α = 0.05).
Toothpaste formulation A showed maximum zones of inhibition against the test organism, Escherichia coli (P<60;0.001) compared to all other toothpastes formulations. Against Streptococcus mutans and Candida albicans, the zones of inhibition were less in comparison to E.coli but were significantly different at higher dilutions (1:8, 1:16 P<60;0.05) for toothpaste formulation A. Mouthrinses formulation H showed maximum efficacy against the test organism, Escherichia coli (P<60;0.001) compared to all other mouthrinse formulations. Against Streptococcus mutans, mouthrinses formulations F, G and J showed significant antimicrobial activity (P<60;0.05) compared to formulation H and I.
In the present study, it has been demonstrated that triclosan containing toothpastes formulations are more effective in control of oral microflora compared to non-triclosan containing synthetic toothpastes. Among mouthrinses formulations, chlorhexidine was found to be more effective than or as effective as triclosan against the organisms tested.
抗菌剂已被用作一种化疗药物来改善口腔健康。进行这项体外研究以确定不同牙膏和漱口水对口腔病原体的抗菌效果。
通过琼脂扩散法，对五种牙膏和五种漱口水针对三种口腔病原体，即变形链球菌（MTCC 890）、大肠杆菌（MTCC 579）和白色念珠菌（MTCC 854）的抗菌活性进行了测试。使用统计软件包SPSS windows版本15进行统计分析，采用方差分析（ANOVA）及事后最小二乘差异（LSD）法计算平均值（α = 0.05）。
与所有其他牙膏配方相比，牙膏配方A对测试生物体大肠杆菌显示出最大抑菌圈（P<60;0.001）。对于变形链球菌和白色念珠菌，与大肠杆菌相比抑菌圈较小，但在较高稀释度（1:8、1:16，P<60;0.05）下，牙膏配方A的抑菌圈有显著差异。漱口水配方H与所有其他漱口水配方相比，对测试生物体大肠杆菌显示出最大功效（P<60;0.001）。对于变形链球菌，漱口水配方F、G和J与配方H和I相比显示出显著的抗菌活性（P<60;0.05）。
在本研究中，已证明含三氯生的牙膏配方在控制口腔微生物群方面比不含三氯生的合成牙膏更有效。在漱口水配方中，发现洗必泰对测试生物体的效果比三氯生更有效或与之相当。
