Adwan Ghaleb, Salameh Yousef, Adwan Kamel, Barakat Ali
Department of Biology and Biotechnology, An-Najah N. University, P. O. Box (7)-Nablus, Palestine.
Asian Pac J Trop Biomed. 2012 May;2(5):375-9. doi: 10.1016/S2221-1691(12)60059-8.
To detect the anticandidal activity of nine toothpastes containing sodium fluoride, sodium monofluorophosphate and herbal extracts as an active ingredients against 45 oral and non oral Candida albicans (C. albicans) isolates.
The antifungal activity of these toothpaste formulations was determined using a standard agar well diffusion method. Statistical analysis was performed using a statistical package, SPSS windows version 15, by applying mean values using one-way ANOVA with post-hoc least square differences (LSD) method. A P value of less than 0.05 was considered significant.
All toothpastes studied in our experiments were effective in inhibiting the growth of all C. albicans isolates. The highest anticandidal activity was obtained from toothpaste that containing both herbal extracts and sodium fluoride as active ingredients, while the lowest activity was obtained from toothpaste containing sodium monofluorophosphate as an active ingredient. Antifungal activity of Parodontax toothpaste showed a significant difference (P< 0.001) against C. albicans isolates compared to toothpastes containing sodium fluoride or herbal products.
In the present study, it has been demonstrated that toothpaste containing both herbal extracts and sodium fluoride as active ingredients are more effective in control of C. albicans, while toothpaste that containing monofluorophosphate as an active ingredient is less effective against C. albicans. Some herbal toothpaste formulations studied in our experiments, appear to be equally effective as the fluoride dental formulations and it can be used as an alternative to conventional formulations for individuals who have an interest in naturally-based products. Our results may provide invaluable information for dental professionals.
检测九种含有氟化钠、单氟磷酸钠和草药提取物作为活性成分的牙膏对45株口腔和非口腔白色念珠菌（白色念珠菌）分离株的抗念珠菌活性。
采用标准琼脂孔扩散法测定这些牙膏配方的抗真菌活性。使用统计软件包SPSS 15.0 for Windows进行统计分析，应用单向方差分析及事后最小二乘差异（LSD）法计算平均值。P值小于0.05被认为具有统计学意义。
我们实验中研究的所有牙膏均能有效抑制所有白色念珠菌分离株的生长。抗念珠菌活性最高的牙膏是同时含有草药提取物和氟化钠作为活性成分的牙膏，而活性最低的是含有单氟磷酸钠作为活性成分的牙膏。与含氟化钠或草药产品的牙膏相比，帕罗顿牙膏对白色念珠菌分离株的抗真菌活性有显著差异（P<0.001）。
在本研究中，已证明同时含有草药提取物和氟化钠作为活性成分的牙膏在控制白色念珠菌方面更有效，而含有单氟磷酸钠作为活性成分的牙膏对白色念珠菌的效果较差。我们实验中研究的一些草药牙膏配方似乎与含氟牙科配方同样有效，对于对天然产品感兴趣的个体，它可以用作传统配方的替代品。我们的结果可能为牙科专业人员提供宝贵的信息。
