Cook G S, Costerton J W, Lamont R J
Center for Biofilm Engineering, Montana State University, Bozeman 59717, USA.
J Periodontal Res. 1998 Aug;33(6):323-7. doi: 10.1111/j.1600-0765.1998.tb02206.x.
Confocal scanning laser microscopy (CSLM) was used to visualize and quantify biofilm formation by the oral bacteria Streptococcus gordonii and Porphyromonas gingivalis. A saliva-coated glass coverslip under continuous bacterial challenge and conditions of low shear force was used to investigate attachment to the salivary pellicle and also the effect of cell-cell interactions on the extent of colonization and biofilm development. S. gordonii bound to the salivary pellicle and outcompeted P. gingivalis for attachment sites. Both P. gingivalis and S. gordonii failed to establish substantial biofilm formation independently. However, biofilm formation did occur subsequent to initial adherence of P. gingivalis to S. gordonii cells deposited on the salivary pellicle. The commensal species S. gordonii may, therefore, provide an attachment substrate for colonization and biofilm accretion by the potential pathogen, P. gingivalis.
共聚焦扫描激光显微镜（CSLM）用于观察和量化口腔细菌戈登氏链球菌和牙龈卟啉单胞菌形成生物膜的情况。在持续细菌攻击和低剪切力条件下，使用涂有唾液的玻璃盖玻片来研究细菌对唾液薄膜的附着情况，以及细胞间相互作用对定植程度和生物膜形成的影响。戈登氏链球菌附着于唾液薄膜，并在附着位点上胜过牙龈卟啉单胞菌。牙龈卟啉单胞菌和戈登氏链球菌都无法独立形成大量生物膜。然而，在牙龈卟啉单胞菌最初附着于沉积在唾液薄膜上的戈登氏链球菌细胞之后，确实会发生生物膜形成。因此，共生菌戈登氏链球菌可能为潜在病原体牙龈卟啉单胞菌的定植和生物膜积聚提供附着底物。
