Mikkelsen Tina B, Osler Merete, Olsen Sjurdur F
Department of Social Medicine, Institute of Public Health, University of Copenhagen, Denmark.
Public Health Nutr. 2006 Sep;9(6):771-8. doi: 10.1079/phn2005883.
To validate intakes of protein, folic acid, retinol and n-3 fatty acids estimated from a food-frequency questionnaire in week 25 of pregnancy (FFQ-25).
Validation was done against a 7-day weighed food diary (FD) and biomarkers of the nutrients in gestation week 32-38.
The FFQ-25 to be validated was used in the Danish National Birth Cohort comprising 101 042 pregnant Danish women, of whom 88 participated in the present validation study.
Estimated intakes of protein, retinol and folic acid did not differ significantly between the two dietary methods, but intake of n-3 fatty acids was one third larger when estimated from the FFQ-25. The intakes estimated from the two dietary methods were all significantly correlated, ranging from 0.20 for retinol intake to 0.57 for folic acid intake. Sensitivities of being correctly classified into low and high quintiles were between 0.22 and 0.77, and specificities were between 0.62 and 0.89. Urinary protein content did not correlate significantly with protein estimated from the FFQ (r = 0.17, P > 0.05), but did with intake estimated from the FD (r = 0.56, P < 0.0001). Erythrocyte folate correlated significantly with the estimated total intake from the FFQ (r = 0.55, P < 0.0001) and the FD (r = 0.52, P < 0.0001). No correlations with plasma retinol were found. Erythrocyte eicosapentaenoic acid (C20:5n-3) correlated significantly with n-3 fatty acids intake estimated from both the FFQ-25 (r = 0.37, P < 0.001) and the FD (r = 0.62, P < 0.0001).
The FFQ-25 gives reasonable valid estimates of protein, retinol and folic acid intakes, but seems to overestimate intake of n-3 fatty acids.
验证通过孕期第25周食物频率问卷（FFQ - 25）估算的蛋白质、叶酸、视黄醇和n - 3脂肪酸摄入量。
以7天称重食物日记（FD）和妊娠第32 - 38周营养素生物标志物为对照进行验证。
用于验证的FFQ - 25应用于丹麦国家出生队列研究，该队列包含101042名丹麦孕妇，其中88名参与了本验证研究。
两种膳食方法估算的蛋白质、视黄醇和叶酸摄入量无显著差异，但通过FFQ - 25估算的n - 3脂肪酸摄入量高出三分之一。两种膳食方法估算的摄入量均显著相关，视黄醇摄入量的相关性为0.20，叶酸摄入量的相关性为0.57。正确分类为低和高五分位数的敏感度在0.22至0.77之间，特异度在0.62至0.89之间。尿蛋白含量与FFQ估算的蛋白质无显著相关性（r = 0.17，P > 0.05），但与FD估算的摄入量显著相关（r = 0.56，P < 0.0001）。红细胞叶酸与FFQ估算的总摄入量显著相关（r = 0.55，P < 0.0001），与FD估算的摄入量也显著相关（r = 0.52，P < 0.0001）。未发现与血浆视黄醇的相关性。红细胞二十碳五烯酸（C20:5n - 3）与FFQ - 25估算的n - 3脂肪酸摄入量显著相关（r = 0.37，P < 0.001），与FD估算的摄入量也显著相关（r = 0.62，P < 0.0001）。
FFQ - 25对蛋白质、视黄醇和叶酸摄入量给出了合理有效的估算，但似乎高估了n - 3脂肪酸的摄入量。
