Mukherjee B B, Mobry P M
J Gen Virol. 1975 Jul;28(1):129-35. doi: 10.1099/0022-1317-28-1-129.
Several adult tissues, newborns, and embryos of uninfected BALB/c mice were analysed for RNA complementary to [3H]-DNA transcripts synthesized from an endogenous type-C virus of BALB/c 3T3. The technique of RNA:DNA hybridization was used and the extent of hybridization was measured by the use of a single-strand-specific nuclease (S-I), purified from Aspergillus oryzae. Virus-specific RNA was detected in all adult and embryonic tissues tested. However, the RNA extracted from tissues having higher proliferative activity, such as spleen, small intestine, uterus and embryos, hybridize the [3H]-DNA probe to a greater extent than the RNA from tissues with low proliferative activity, such as kidney and liver. These observations add further support to the view that the repression of the virus genome in normal cells is not complete, and suggest the existence of a correlation between a qualitative or quantitative change in the endogenous C-type virus genome transcription pattern and cell proliferation.
对未感染的BALB/c小鼠的几种成体组织、新生小鼠和胚胎进行了分析，以检测与从BALB/c 3T3细胞的内源性C型病毒合成的[3H] - DNA转录本互补的RNA。采用RNA:DNA杂交技术，并使用从米曲霉中纯化的单链特异性核酸酶（S-I）来测量杂交程度。在所有测试的成体和胚胎组织中均检测到病毒特异性RNA。然而，从具有较高增殖活性的组织（如脾脏、小肠、子宫和胚胎）中提取的RNA与[3H] - DNA探针的杂交程度比从增殖活性较低的组织（如肾脏和肝脏）中提取的RNA更大。这些观察结果进一步支持了正常细胞中病毒基因组抑制不完全的观点，并表明内源性C型病毒基因组转录模式的定性或定量变化与细胞增殖之间存在相关性。
