Mukherjee B B, Mobry P M, Vincent R N
J Gen Virol. 1980 Aug;49(2):411-6. doi: 10.1099/0022-1317-49-2-411.
Analysis of endogenous X-tropic BALB/c retrovirus-specific RNAs in a BALB/c mouse tissue containing mostly non-driving cells (liver), in two normal tissues having significant proportions of dividing cells (20-h regenerating liver and 12-day embryo) and in cells of a lymphosarcoma of BALB/c mice were carried out by determining the extent to which the RNAs from these tissues hybridized to the virus 3H-cDNA probe and by their relative sedimentation values in a sucrose gradient. RNAs from 20-h regenerating liver, 12-day embryo and lymphosarcoma, each containing a significant proportion of proliferating cells, showed 8 to 21 % higher hybridization values than normal liver RNA. Differences in the exact size classes of virus-specific RNAs and, in their relative proportions were found to exist in the four different tissue types examined and no correlation between a specific RNA size-profile and the proliferating activity of a tissue could be detected.
通过测定来自这些组织的RNA与病毒3H - cDNA探针杂交的程度以及它们在蔗糖梯度中的相对沉降值，对BALB / c小鼠主要含非增殖细胞的组织（肝脏）、含大量分裂细胞的两种正常组织（20小时再生肝脏和12天胚胎）以及BALB / c小鼠淋巴肉瘤细胞中的内源性X - 嗜性BALB / c逆转录病毒特异性RNA进行了分析。来自20小时再生肝脏、12天胚胎和淋巴肉瘤的RNA，每种都含有相当比例的增殖细胞，其杂交值比正常肝脏RNA高8%至21%。在所检测的四种不同组织类型中，发现病毒特异性RNA的确切大小类别及其相对比例存在差异，并且未检测到特定RNA大小谱与组织增殖活性之间的相关性。
