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弗鲁什卡山三种木贼属植物的自由基清除活性。

Free radical scavenging activity of three Equisetum species from Fruska gora mountain.

作者信息

Stajner D, Popović B M, Canadanović-Brunet J, Boza P

机构信息

Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia and Montenegro.

出版信息

Fitoterapia. 2006 Dec;77(7-8):601-4. doi: 10.1016/j.fitote.2006.06.006. Epub 2006 Jul 6.

Abstract

The scavenger activities of Equisetum arvense, Equisetum ramosissimum and Equisetum telmateia aboveground parts phosphate buffer (pH 7) extracts were evaluated using three different methods: DPPH assay, ESR and NO radical inhibition assay. Total reducing power was determined by FRAP assay. The E. telmateia extract demonstrated the most relevant scavenger and antioxidant properties. ESR signal of DMPO-OH radical adducts in the presence of E. telmateia phosphate buffer (pH 7) extract was reduced to 98.9% which indicated that E. telmateia could be a useful source of antioxidants with huge scavenger ability.

摘要

利用三种不同方法评估了问荆、节节草和西洋木贼地上部分磷酸盐缓冲液(pH 7)提取物的清除活性:DPPH 测定法、电子自旋共振(ESR)和一氧化氮自由基抑制测定法。通过铁离子还原抗氧化能力(FRAP)测定法测定总还原能力。西洋木贼提取物表现出最显著的清除和抗氧化特性。在西洋木贼磷酸盐缓冲液(pH 7)提取物存在的情况下,DMPO-OH 自由基加合物的 ESR 信号降低至 98.9%,这表明西洋木贼可能是具有巨大清除能力的抗氧化剂的有用来源。

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