Lewis M H, Gluck J P, Beauchamp A J, Keresztury M F, Mailman R B
Department of Psychiatry, University of North Carolina School of Medicine, Chapel Hill 27599.
Brain Res. 1990 Apr 9;513(1):67-73. doi: 10.1016/0006-8993(90)91089-y.
The hypothesis that early social isolation results in long-term alterations in dopamine receptor sensitivity was tested using older adult rhesus monkeys. Isolated and control monkeys were challenged with apomorphine (0.1 and 0.3 mg/kg), and the drug effects on spontaneous blink rate, stereotyped behavior, and self-injurious behavior were quantified using observational measures. Monoamine metabolites were quantified from cisternal CSF by HPLC-EC, prior to pharmacological challenge. Isolated and control monkeys did not differ in CSF concentrations of HVA, 5-HIAA, or MHPG. At the higher dose, apomorphine significantly increased the rate of blinking, the occurrence of whole-body stereotypies, and the intensity of stereotyped behavior (as measured by observer ratings) in isolated monkeys. The frequency of occurrence of self-injurious behavior was too low to allow for meaningful comparisons. These significant differences in response to apomorphine challenge support the hypothesis that long-term or permanent alterations in dopamine receptor sensitivity, as assessed by drug challenge, are a consequence of early social deprivation.
使用成年恒河猴检验了早期社会隔离会导致多巴胺受体敏感性长期改变这一假说。对隔离饲养的猴子和对照猴子注射阿扑吗啡（0.1和0.3毫克/千克），并通过观察测量来量化药物对自发眨眼频率、刻板行为和自伤行为的影响。在进行药物激发之前，通过高效液相色谱-电化学检测法对脑池脑脊液中的单胺代谢产物进行定量分析。隔离饲养的猴子和对照猴子的脑脊液中高香草酸（HVA）、5-羟吲哚乙酸（5-HIAA）或3-甲氧基-4-羟基苯乙二醇（MHPG）的浓度没有差异。在较高剂量下，阿扑吗啡显著增加了隔离饲养猴子的眨眼频率、全身刻板行为的发生率以及刻板行为的强度（通过观察者评分衡量）。自伤行为的发生频率过低，无法进行有意义的比较。这些对阿扑吗啡激发反应的显著差异支持了这样一种假说，即通过药物激发评估的多巴胺受体敏感性的长期或永久性改变是早期社会剥夺的结果。
