Liprandi F, Lopez G, Rodriguez I, Hidalgo M, Ludert J E, Mattion N
Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela.
J Gen Virol. 1990 Jun;71 ( Pt 6):1395-8. doi: 10.1099/0022-1317-71-6-1395.
A panel of 10 monoclonal antibodies produced after immunization with two porcine subgroup I rotavirus strains (OSU and A46), and directed against the major inner capsid protein (VP6), fell into six patterns of reactivity when tested against a collection of human and animal group A rotavirus strains. Monoclonal antibodies of pattern I recognized all rotavirus strains. Antibodies of patterns 2 and 3 recognized all subgroup II strains and some, but not all, subgroup I strains. Pattern 4 antibodies identified all subgroup I strains and two strains (H2, equine; CC117, porcine) not reactive with reference subgroup monoclonal antibodies (strains non-I non-II). Pattern 5 antibody exhibited the same reactivity as pattern 4 except for not recognizing the non-I non-II equine strain. Pattern 6 antibodies reacted exclusively with subgroup I and non-I non-II rotaviruses of porcine origin. By competitive binding assays, monoclonal antibodies of patterns 4, 5 and 6 appeared to recognize a single antigenic site, which included at least three overlapping epitopes. In immunoblots all monoclonal antibodies, except one, recognized only the trimeric, but not the monomeric form of VP6.
用两种猪I群轮状病毒株（OSU和A46）免疫后产生的一组10种单克隆抗体，针对主要内衣壳蛋白（VP6），在针对一组人和动物A群轮状病毒株进行测试时，呈现出六种反应模式。I型单克隆抗体识别所有轮状病毒株。2型和3型抗体识别所有II群菌株以及一些（但不是全部）I群菌株。4型抗体识别所有I群菌株以及两种与参考亚群单克隆抗体无反应的菌株（H2，马；CC117，猪）（非I非II型菌株）。5型抗体表现出与4型相同的反应性，只是不识别非I非II型马源菌株。6型抗体仅与猪源I群和非I非II型轮状病毒反应。通过竞争性结合试验，4型、5型和6型单克隆抗体似乎识别一个单一的抗原位点，该位点至少包括三个重叠表位。在免疫印迹中，除一种外，所有单克隆抗体仅识别VP6的三聚体形式，而不识别单体形式。
