Minamoto N, Sugimoto O, Yokota M, Tomita M, Goto H, Sugiyama M, Kinjo T
Department of Veterinary Public Health, Faculty of Agriculture, Gifu University, Japan.
Arch Virol. 1993;131(3-4):293-305. doi: 10.1007/BF01378633.
Monoclonal antibodies (MAbs) were prepared to analyze antigens on the major inner capsid protein, VP6 of avian group A rotavirus. Based on the results of a competitive binding assay using 15 MAbs directed against VP6 of the PO-13 rotavirus strain, isolated from a pigeon in Japan, it was found that VP6 of avian rotavirus possesses at least four spatially distinct antigenic sites. Two antigenic sites (I and II) were topologically distinct from the other two (III and IV), which were in close proximity. From the reaction of MAbs in indirect immunofluorescent antibody tests to a series of known rotaviruses, epitopes representing common antigens of all group A rotavirus including avian rotavirus were localized in sites II and III. One epitope in site IV appeared to have a subgroup antigenic specificity that reacted only with rotaviruses belonging to subgroup I. Interestingly, avian rotaviruses isolated from turkeys and chickens in Northern Ireland also reacted only with these subgroup I specific MAbs, but not with subgroup II specific MAb. This indicates that avian rotavirus has subgroup I specific antigen, which is antigenically similar to that of other mammalian rotavirus strains.
制备单克隆抗体（MAb）以分析禽A组轮状病毒主要内衣壳蛋白VP6上的抗原。基于使用针对从日本一只鸽子分离的PO - 13轮状病毒株VP6的15种单克隆抗体进行的竞争性结合试验结果，发现禽轮状病毒的VP6具有至少四个空间上不同的抗原位点。两个抗原位点（I和II）在拓扑结构上与另外两个紧密相邻的位点（III和IV）不同。通过单克隆抗体在间接免疫荧光抗体试验中与一系列已知轮状病毒的反应，代表包括禽轮状病毒在内的所有A组轮状病毒共同抗原的表位定位于位点II和III。位点IV中的一个表位似乎具有亚组抗原特异性，仅与属于亚组I的轮状病毒反应。有趣的是，从北爱尔兰的火鸡和鸡中分离的禽轮状病毒也仅与这些亚组I特异性单克隆抗体反应，而不与亚组II特异性单克隆抗体反应。这表明禽轮状病毒具有亚组I特异性抗原，其在抗原性上与其他哺乳动物轮状病毒株相似。
