Department of Food Technology Engineering and Nutrition, Lund University, Box 124, 22100 Lund, Sweden.
The Diabetes and Celiac Disease Unit, Department of Clinical Sciences, Lund University, 21428 Malmö, Sweden.
Nutrients. 2019 Aug 16;11(8):1925. doi: 10.3390/nu11081925.
Two strains have proven anti-inflammatory properties by reducing pro-inflammatory responses to antigens. This randomized double-blind placebo-controlled trial tested the hypothesis that HEAL9 and 8700:2 suppress ongoing celiac disease autoimmunity in genetically at risk children on a gluten-containing diet in a longitudinally screening study for celiac disease. Seventy-eight children with celiac disease autoimmunity participated of whom 40 received 10 CFU/day of HEAL9 and 8700:2 (probiotic group) and 38 children maltodextrin (placebo group) for six months. Blood samples were drawn at zero, three and six months and phenotyping of peripheral blood lymphocytes and IgA and IgG autoantibodies against tissue transglutaminase (tTG) were measured. In the placebo group, naïve CD45RA+ Th cells decreased ( = 0.002) whereas effector and memory CD45RO+ Th cells increased ( = 0.003). In contrast, populations of cells expressing CD4+CD25CD45RO+CCR4+ increased in the placebo group ( = 0.001). Changes between the groups were observed for NK cells ( = 0.038) and NKT cells ( = 0.008). Median levels of IgA-tTG decreased more significantly over time in the probiotic ( = 0.013) than in the placebo ( = 0.043) group whereas the opposite was true for IgG-tTG ( = 0.062 respective = 0.008). In conclusion, daily oral administration of HEAL9 and 8700:2 modulate the peripheral immune response in children with celiac disease autoimmunity.
两种菌株已被证明具有抗炎特性，可减少对抗原的促炎反应。这项随机、双盲、安慰剂对照试验检验了以下假设：在一项针对乳糜泻的纵向筛查研究中，对于饮食中含有麸质且存在遗传风险的儿童，每天摄入 10 亿 CFU 的 HEAL9 和 8700:2（益生菌组）可抑制正在进行的乳糜泻自身免疫，HEAL9 和 8700:2 可抑制遗传易感儿童的乳糜泻自身免疫。共有 78 名乳糜泻自身免疫患儿参与了研究，其中 40 名患儿接受了 10 亿 CFU/天的 HEAL9 和 8700:2（益生菌组），38 名患儿接受了麦芽糊精（安慰剂组），为期 6 个月。在零、三、六个月时采集血样，并检测外周血淋巴细胞表型和针对组织转谷氨酰胺酶（tTG）的 IgA 和 IgG 自身抗体。在安慰剂组中，幼稚 CD45RA+Th 细胞减少（ = 0.002），而效应和记忆 CD45RO+Th 细胞增加（ = 0.003）。相比之下，在安慰剂组中，表达 CD4+CD25CD45RO+CCR4+的细胞群增加（ = 0.001）。NK 细胞（ = 0.038）和 NKT 细胞（ = 0.008）之间的变化也在两组间观察到。益生菌组中 IgA-tTG 的中位数水平随时间的推移下降更明显（ = 0.013），而安慰剂组中 IgG-tTG 的中位数水平下降更明显（ = 0.043），而 IgG-tTG 的情况则相反（ = 0.062 分别为 = 0.008）。总之，每天口服 HEAL9 和 8700:2 可调节乳糜泻自身免疫患儿的外周免疫反应。
