Kassuba A, Saif L J, Greenberg H B
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
Am J Vet Res. 1990 Jun;51(6):938-44.
Fifty-six samples of feces and intestinal contents from nonvaccinated diarrheal pigs with rotavirus infections were tested, using a subgroup (SGP)-specific ELISA, to determine rotavirus SGP classification. Forty-one percent (23/56) were SGP 1, 25% (14/56) were SGP 2, and 34% (19/56) were not classifiable. For classifiable samples, the geographic distribution for SGP 1 and SGP 2, respectively was: 60%/40% from Ohio (n = 15), 63%/37% from other midwestern states (Iowa, Minnesota, Nebraska, South Dakota; n = 16), and 67%/33% from Canada (n = 6). Thirty-seven SGP-classifiable samples were categorized according to age of pigs. Of pigs less than or equal to 1 week old, 22% of samples were SGP 1 (n = 8), and 14% (n = 5) were SGP 2. Of samples from 1- to 2-week-old pigs, 8% were SGP 1 (n = 3), and 5% were SGP 2 (n = 2). Of samples from 2- to 3-week-old pigs, 5% were SGP 1 (n = 2), and 8% were SGP 2 (n = 3). Of samples from 3- to 4-week-old pigs, 5% were SGP 1 (n = 2), and 3% were SGP 2 (n = 1). Of samples from pigs greater than 4 weeks old, 22% were SGP 1 (n = 8) and 8% were SGP 2 (n = 3). Double-stranded RNA extracted from positive controls and from 10 selected field samples (5 from SGP 1 and 5 from SGP 2) was electrophoresed in polyacrylamide gels to detect correlation between subgroup classification by ELISA and long or short double-stranded RNA electrophoretic-migration patterns. All SGP-1 and -2 rotavirus samples tested had typical long double-stranded RNA electrophoretic-migration patterns.
对56份来自未接种疫苗、感染轮状病毒的腹泻猪的粪便和肠道内容物样本，使用亚组（SGP）特异性ELISA进行检测，以确定轮状病毒SGP分类。41%（23/56）为SGP 1，25%（14/56）为SGP 2，34%（19/56）无法分类。对于可分类的样本，SGP 1和SGP 2的地理分布分别为：来自俄亥俄州的占60%/40%（n = 15），来自其他中西部州（爱荷华州、明尼苏达州、内布拉斯加州、南达科他州）的占63%/37%（n = 16），来自加拿大的占67%/33%（n = 6）。37份可进行SGP分类的样本根据猪的年龄进行了分类。在小于或等于1周龄的猪中，22%的样本为SGP 1（n = 8），14%（n = 5）为SGP 2。在1至2周龄猪的样本中，8%为SGP 1（n = 3），5%为SGP 2（n = 2）。在2至3周龄猪的样本中，5%为SGP 1（n = 2），8%为SGP 2（n = 3）。在3至4周龄猪的样本中，5%为SGP 1（n = 2），3%为SGP 2（n = 1）。在大于4周龄猪的样本中，22%为SGP 1（n = 8），8%为SGP 2（n = 3）。从阳性对照和10份选定的田间样本（5份来自SGP 1，5份来自SGP 2）中提取的双链RNA在聚丙烯酰胺凝胶中进行电泳，以检测ELISA亚组分类与双链RNA长或短电泳迁移模式之间的相关性。所有检测的SGP-1和-2轮状病毒样本均具有典型的长双链RNA电泳迁移模式。
