Lucchelli A, Lance S E, Bartlett P B, Miller G Y, Saif L J
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
Am J Vet Res. 1992 Feb;53(2):169-74.
Fecal samples were collected from 450 neonatal calves, ranging from 1 to 30 days old, between May, 1988 and May, 1989 to estimate the prevalence of bovine group A rotavirus in a stratified random sample of Ohio dairy herds. Calves were from 47 dairy herds chosen to be representative of Ohio herds. Bovine group A rotavirus was detected in fecal samples by a cell culture immunofluorescence test (CCIF) and ELISA. Of 450 samples tested, 46 (10%) were positive by CCIF and 67 (15%) were positive by ELISA. The agreement beyond chance between the 2 assays was good (kappa = 0.65). The overall prevalence rate of rotavirus shedding was 16.4% (74/450). Forty-three percent (29/67) of the samples positive by ELISA were subgroup 1, none were subgroup 2, and the remaining 57% (38/67) could not be assigned to either subgroups 1 or 2. Thirty herds (62.5%) had at least 1 group A rotavirus-positive calf (mean number of samples per positive herd = 12.4), and 17 herds (37.5%) had no rotavirus-positive calves (mean number of samples per negative herd = 6.0). A live oral rotacoronavirus vaccine was used in neonatal calves of only 1 herd and 3 of 17 (17.6%) calves from this herd were positive for group A rotavirus. The percentage of the rotavirus-positive fecal samples from all calves (n = 450) when stratified by fecal consistency was as follows: 28.3% (13/46) had liquid feces; 25.6% (10/39) had semiliquid feces; 23.4% (22/94) had pasty feces; and 10.7% (29/271) had firm feces.(ABSTRACT TRUNCATED AT 250 WORDS)
1988年5月至1989年5月期间，从450头1至30日龄的新生犊牛采集粪便样本，以估计俄亥俄州奶牛群分层随机样本中A组牛轮状病毒的流行率。这些犊牛来自47个被选为代表俄亥俄州牛群的奶牛场。通过细胞培养免疫荧光试验（CCIF）和酶联免疫吸附测定（ELISA）检测粪便样本中的A组牛轮状病毒。在检测的450份样本中，CCIF检测阳性的有46份（10%），ELISA检测阳性的有67份（15%）。两种检测方法之间的一致性良好（kappa = 0.65）。轮状病毒排毒的总体流行率为16.4%（74/450）。ELISA检测阳性的样本中，43%（29/67）为1型亚组，无2型亚组，其余57%（38/67）不能归为1型或2型亚组。30个牛群（62.5%）至少有1头A组轮状病毒阳性犊牛（每个阳性牛群的样本平均数 = 12.4），17个牛群（37.5%）没有轮状病毒阳性犊牛（每个阴性牛群的样本平均数 = 6.0）。仅1个牛群的新生犊牛使用了口服活性轮状冠状病毒疫苗，该牛群17头犊牛中有3头（17.6%）A组轮状病毒检测呈阳性。按粪便稠度分层时，所有犊牛（n = 450）中轮状病毒阳性粪便样本的百分比情况如下：稀便的占28.3%（13/46）；半稀便的占25.6%（10/39）；糊状便的占23.4%（22/94）；硬便的占10.7%（29/271）。（摘要截短于250词）
