Lundström A, Egelrud T
Department of Dermatology, University of Umeå, Sweden.
Arch Dermatol Res. 1990;282(4):234-7. doi: 10.1007/BF00371642.
A recently described endogenous proteolytic process in pieces of human plantar stratum corneum incubated in vitro has been further studied. This process leads to a decrease in cohesion between the cells that had been facing outwards in vivo. Using two methods, that differed with respect to efficiency, to detach surface cells with decreased cohesion, the process could be divided into two steps. The first step took place irrespective of the presence of ethylenediaminetetraacetate (EDTA) and led to a moderate decrease in cohesion between surface cells. The second step occurred only in the presence of EDTA and advanced to a point where the surface cells could be separated from the remaining cohesive tissue pieces by simple agitation. Both degradation steps could be inhibited by aprotinin and chymostatin but not by leupeptin. Zinc sulfate inhibited the first step. The results indicate that there are two different types of protein structures being degraded during the process of cell shedding in vitro. A chymotrypsin-like enzyme may be involved in the process.
对最近描述的在体外培养的人足底角质层切片中的一种内源性蛋白水解过程进行了进一步研究。该过程导致体内朝外的细胞之间的黏附力下降。使用两种效率不同的方法来分离黏附力下降的表面细胞，该过程可分为两个步骤。第一步的发生与乙二胺四乙酸（EDTA）的存在无关，导致表面细胞之间的黏附力适度下降。第二步仅在EDTA存在时发生，并进展到通过简单搅拌即可将表面细胞与其余黏附的组织片分离的程度。两个降解步骤均可被抑肽酶和抑糜酶抑制，但不能被亮抑肽酶抑制。硫酸锌抑制第一步。结果表明，在体外细胞脱落过程中有两种不同类型的蛋白质结构被降解。一种类胰凝乳蛋白酶可能参与了该过程。
