Bissett D L, McBride J F, Patrick L F
Arch Dermatol Res. 1987;279(3):184-9. doi: 10.1007/BF00413255.
The work reported here indicates that protein and calcium have roles in stratum corneum cell cohesion. A zwitterionic surfactant, 6-octadecyldimethyl ammoniohexanoate (C18AH), was found to completely disaggregate pig and human stratum corneum into intact, individual cells. This method of disaggregation provided a tool to determine the role of tissue components in cell cohesion. The C18AH disaggregation of pig and human stratum corneum was accelerated by proteolytic enzyme and ethylenediaminetetraacetate (EDTA). The C18AH disaggregation could be blocked by pretreatment of the stratum corneum with the serine-type proteolytic enzyme inhibitor phenylmethylsulfonyl fluoride (PMSF). The blockage could be overcome by addition of proteolytic enzyme or divalent metal ion chelator. These and other data indicate the importance of protein and calcium in stratum corneum cell cohesion.
此处报道的研究表明，蛋白质和钙在角质层细胞黏附中发挥作用。一种两性离子表面活性剂，6-十八烷基二甲基铵己酸盐（C18AH），被发现能将猪和人的角质层完全分解成完整的单个细胞。这种分解方法提供了一种工具，用于确定组织成分在细胞黏附中的作用。猪和人角质层的C18AH分解作用可被蛋白酶和乙二胺四乙酸（EDTA）加速。角质层用丝氨酸型蛋白酶抑制剂苯甲基磺酰氟（PMSF）预处理后，C18AH分解作用可被阻断。添加蛋白酶或二价金属离子螯合剂可克服这种阻断作用。这些及其他数据表明蛋白质和钙在角质层细胞黏附中的重要性。
