花生四烯酸及其他顺式不饱和脂肪酸对分泌性氯离子通道的直接调节作用。
Direct modulation of secretory chloride channels by arachidonic and other cis unsaturated fatty acids.
作者信息
Hwang T C, Guggino S E, Guggino W B
机构信息
Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
出版信息
Proc Natl Acad Sci U S A. 1990 Aug;87(15):5706-9. doi: 10.1073/pnas.87.15.5706.
Abstract
The effect of fatty acids on Cl- channels and transepithelial Cl- secretion is investigated. Patch-clamp experiments show that arachidonic acid blocks Cl- channels in a dose-dependent manner. Kinetic analysis shows that the mean open time is decreased 10-fold with 25 microM arachidonic acid. There is a linear relationship between the reciprocal of mean open time and blocker concentration within the range of 1 to 25 microM. The reciprocal of mean blocked time does not change with arachidonic acid concentration. Other cis unsaturated fatty acids, including oleic, linoleic, and ricinoleic acids, demonstrate similar blocks. Trans unsaturated acids such as elaidic acid and saturated fatty acids, including stearic, palmitic, and myristic acids, do not inhibit the channel at 20 microM. Ricinoleic acid decreases short circuit current in T84 cells, a colonic carcinoma cell line that secretes Cl-. Our results suggest that the direct effect of arachidonic and other fatty acids on Cl- secretion is to block Cl- channel current.
摘要
研究了脂肪酸对氯离子通道和跨上皮氯离子分泌的影响。膜片钳实验表明，花生四烯酸以剂量依赖方式阻断氯离子通道。动力学分析显示，25微摩尔花生四烯酸可使平均开放时间缩短10倍。在1至25微摩尔范围内，平均开放时间的倒数与阻滞剂浓度之间存在线性关系。平均阻断时间的倒数不随花生四烯酸浓度变化。其他顺式不饱和脂肪酸，包括油酸、亚油酸和蓖麻油酸，也表现出类似的阻断作用。反式不饱和酸如反油酸以及饱和脂肪酸，包括硬脂酸、棕榈酸和肉豆蔻酸，在20微摩尔时不抑制该通道。蓖麻油酸可降低T84细胞（一种分泌氯离子的结肠癌细胞系）的短路电流。我们的结果表明，花生四烯酸和其他脂肪酸对氯离子分泌的直接作用是阻断氯离子通道电流。
相似文献
1
Direct modulation of secretory chloride channels by arachidonic and other cis unsaturated fatty acids.花生四烯酸及其他顺式不饱和脂肪酸对分泌性氯离子通道的直接调节作用。
Proc Natl Acad Sci U S A. 1990 Aug;87(15):5706-9. doi: 10.1073/pnas.87.15.5706.
2
Fatty acids inhibit apical membrane chloride channels in airway epithelia.脂肪酸抑制气道上皮细胞顶端膜氯化物通道。
Proc Natl Acad Sci U S A. 1990 Sep;87(18):7334-8. doi: 10.1073/pnas.87.18.7334.
3
Modulation of K+ channels by arachidonic acid in T84 cells. I. Inhibition of the Ca(2+)-dependent K+ channel.花生四烯酸对T84细胞中钾离子通道的调节作用。I. 对钙依赖性钾离子通道的抑制作用
Am J Physiol. 1998 Jan;274(1):C138-48. doi: 10.1152/ajpcell.1998.274.1.C138.
4
Regulation of human placental chloride channel by arachidonic acid and other cis unsaturated fatty acids.花生四烯酸及其他顺式不饱和脂肪酸对人胎盘氯通道的调节作用。
Am J Obstet Gynecol. 1999 Feb;180(2 Pt 1):469-75. doi: 10.1016/s0002-9378(99)70234-6.
5
Biophysical properties of a chloride channel in the apical membrane of a secretory epithelial cell.分泌性上皮细胞顶端膜中氯离子通道的生物物理特性。
Comp Biochem Physiol A Comp Physiol. 1988;90(4):597-601. doi: 10.1016/0300-9629(88)90673-1.
6
Apical membrane Cl- channels in airway epithelia: anion selectivity and effect of an inhibitor.气道上皮细胞顶端膜氯离子通道：阴离子选择性及一种抑制剂的作用
Am J Physiol. 1990 Aug;259(2 Pt 1):C295-301. doi: 10.1152/ajpcell.1990.259.2.C295.
7
Modulation of voltage-dependent Ca channel current by arachidonic acid and other long-chain fatty acids in rabbit intestinal smooth muscle.花生四烯酸及其他长链脂肪酸对兔肠平滑肌电压依赖性钙通道电流的调节作用
J Gen Physiol. 1992 Jul;100(1):27-44. doi: 10.1085/jgp.100.1.27.
8
Modulation of native TREK-1 and Kv1.4 K+ channels by polyunsaturated fatty acids and lysophospholipids.多不饱和脂肪酸和溶血磷脂对天然TREK-1和Kv1.4钾通道的调节作用。
J Membr Biol. 2003 Oct 1;195(3):147-64. doi: 10.1007/s00232-003-0616-0.
9
Two types of chloride channel on duct cells cultured from human fetal pancreas.从人胎儿胰腺培养的导管细胞上的两种氯离子通道。
Am J Physiol. 1989 Aug;257(2 Pt 1):C240-51. doi: 10.1152/ajpcell.1989.257.2.C240.
10
Direct activation of purified protein kinase C by unsaturated fatty acids (oleate and arachidonate) in the absence of phospholipids and Ca2+.在不存在磷脂和钙离子的情况下，不饱和脂肪酸（油酸和花生四烯酸）对纯化的蛋白激酶C的直接激活作用。
FEBS Lett. 1985 Nov 18;192(2):189-93. doi: 10.1016/0014-5793(85)80105-8.
引用本文的文献
1
A Fatty Acids Mixture Reduces Anxiety-Like Behaviors in Infant Rats Mediated by GABA Receptors.脂肪酸混合物通过 GABA 受体减少幼鼠的焦虑样行为。
Biomed Res Int. 2017;2017:8798546. doi: 10.1155/2017/8798546. Epub 2017 Dec 17.
2
Actions and Mechanisms of Polyunsaturated Fatty Acids on Voltage-Gated Ion Channels.多不饱和脂肪酸对电压门控离子通道的作用及机制
Front Physiol. 2017 Feb 6;8:43. doi: 10.3389/fphys.2017.00043. eCollection 2017.
3
Myristic acid produces anxiolytic-like effects in Wistar rats in the elevated plus maze.肉豆蔻酸在高架十字迷宫实验中对Wistar大鼠产生抗焦虑样作用。
Biomed Res Int. 2014;2014:492141. doi: 10.1155/2014/492141. Epub 2014 Sep 18.
4
Sensory TRP channel interactions with endogenous lipids and their biological outcomes.感觉性瞬时受体电位（TRP）通道与内源性脂质的相互作用及其生物学结果。
Molecules. 2014 Apr 15;19(4):4708-44. doi: 10.3390/molecules19044708.
5
Analysis by fast-atom bombardment tandem mass spectrometry of phosphatidylcholine isolated from heart mitochondrial fractions: Evidence of incorporation of monohydroxylated fatty acyl moieties.用快原子轰击串联质谱分析法分析心肌线粒体部分分离出的磷脂酰胆碱：单羟基脂肪酸酯成分掺入的证据。
J Am Soc Mass Spectrom. 1996 Jan;7(1):50-8. doi: 10.1016/1044-0305(95)00623-0.
6
Participation of GABAA chloride channels in the anxiolytic-like effects of a fatty acid mixture.参与 GABA A 氯通道在脂肪酸混合物抗焦虑样作用中的作用。
Biomed Res Int. 2013;2013:121794. doi: 10.1155/2013/121794. Epub 2013 Sep 19.
7
Amniotic fluid or its fatty acids produce actions similar to diazepam on lateral septal neurons firing rate.羊水及其脂肪酸对外侧隔区神经元的放电频率产生与地西泮相似的作用。
ScientificWorldJournal. 2013 Jun 24;2013:534807. doi: 10.1155/2013/534807. Print 2013.
8
Anxiolytic-like actions of fatty acids identified in human amniotic fluid.在人羊水中鉴定出的脂肪酸的抗焦虑样作用。
ScientificWorldJournal. 2013 Apr 30;2013:823289. doi: 10.1155/2013/823289. Print 2013.
9
A dual action of saturated fatty acids on electrical activity in rat pancreatic β-cells. Role of volume-regulated anion channel and KATP channel currents.饱和脂肪酸对大鼠胰岛β细胞电活动的双重作用。容积调节阴离子通道和 KATP 通道电流的作用。
J Physiol. 2011 Mar 15;589(Pt 6):1307-16. doi: 10.1113/jphysiol.2010.200741. Epub 2011 Jan 17.
10
Potentiation of EDHF-mediated relaxation by chloride channel blockers.氯离子通道阻断剂增强 EDHF 介导的舒张作用。
Acta Pharmacol Sin. 2010 Oct;31(10):1303-11. doi: 10.1038/aps.2010.157. Epub 2010 Sep 13.
本文引用的文献
1
Bronchoconstrictor effects of leukotriene C in humans.白三烯C对人体的支气管收缩作用。
Science. 1982 Apr 9;216(4542):196-8. doi: 10.1126/science.7063880.
2
How is the level of free arachidonic acid controlled in mammalian cells?哺乳动物细胞中游离花生四烯酸的水平是如何控制的？
Biochem J. 1982 Apr 15;204(1):3-16. doi: 10.1042/bj2040003.
3
Stimulation of colonic secretion by lipoxygenase metabolites of arachidonic acid.花生四烯酸的脂氧合酶代谢产物对结肠分泌的刺激作用。
Science. 1982 Sep 24;217(4566):1255-6. doi: 10.1126/science.6810465.
4
Prostaglandin D2 generation after activation of rat and human mast cells with anti-IgE.用抗IgE激活大鼠和人类肥大细胞后前列腺素D2的生成
J Immunol. 1982 Oct;129(4):1627-31.
5
Activation of multiple-conductance state chloride channels in spinal neurones by glycine and GABA.甘氨酸和γ-氨基丁酸对脊髓神经元中多电导态氯离子通道的激活作用。
Nature. 1983;305(5937):805-8. doi: 10.1038/305805a0.
6
Single voltage-dependent chloride-selective channels of large conductance in cultured rat muscle.培养的大鼠肌肉中具有大电导的单电压依赖性氯选择性通道。
Biophys J. 1983 Aug;43(2):237-41. doi: 10.1016/S0006-3495(83)84344-6.
7
Allergen challenge of lung tissue from asthmatics elicits bronchial contraction that correlates with the release of leukotrienes C4, D4, and E4.对哮喘患者肺组织进行变应原激发会引发支气管收缩，这与白三烯C4、D4和E4的释放相关。
Proc Natl Acad Sci U S A. 1983 Mar;80(6):1712-6. doi: 10.1073/pnas.80.6.1712.
8
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.用于从细胞和无细胞膜片进行高分辨率电流记录的改进膜片钳技术。
Pflugers Arch. 1981 Aug;391(2):85-100. doi: 10.1007/BF00656997.
9
Activation of guinea pig pulmonary adenylate cyclase by prostacyclin.前列环素对豚鼠肺腺苷酸环化酶的激活作用。
Eur J Pharmacol. 1980 Oct 31;67(4):419-25. doi: 10.1016/0014-2999(80)90183-1.
10
A potential second messenger role for unsaturated fatty acids: activation of Ca2+-dependent protein kinase.不饱和脂肪酸可能的第二信使作用：钙依赖性蛋白激酶的激活。
Science. 1984 May 11;224(4649):622-5. doi: 10.1126/science.6231726.
Zotero 插件