Arabatzis Michael, Xylouri Eftihia, Frangiadaki Irene, Tzimogianni Aleka, Milioni Afroditi, Arsenis George, Velegraki Aristea
Mycology Laboratory, Department of Microbiology, Medical School, University of Athens, Athens, Greece.
Vet Dermatol. 2006 Oct;17(5):322-6. doi: 10.1111/j.1365-3164.2006.00535.x.
Three commercial rabbit farms independently reported suspected dermatophyte infections during 2004. Cultures confirmed that the infection was caused by the zoophilic Tricophyton mentagrophytes, while mating studies and sequencing of the internal transcribed spacer (ITS)1/4 polymerase chain reaction products, directly amplified from skin lesions and from pure culture isolates, established its Arthroderma vanbreuseghemii lineage. Subsequently, the corresponding DdeI restriction profiles of the ITS1/4 PCR products matched one of the two published T. mentagrophytes complex profiles. Sequence analysis of the published ITS sequences predicted distinct DdeI restriction sites for Arthroderma benhamiae and A. vanbreuseghemii, while all DdeI-generated restriction fragment length polymorphisms of the rabbit isolates were identical to those of A. vanbreuseghemii. The proposed procedure can be used for rapid dermatophyte detection and presumptive T. mentagrophytes identification.
2004年，三家商业养兔场独立报告了疑似皮肤癣菌感染病例。培养结果证实，感染是由亲动物性须癣毛癣菌引起的，而通过交配研究以及对直接从皮肤损伤处和纯培养分离物中扩增的内转录间隔区（ITS）1/4聚合酶链反应产物进行测序，确定其为梵氏节皮菌谱系。随后，ITS1/4 PCR产物相应的DdeI限制性酶切图谱与已发表的两种须癣毛癣菌复合图谱之一相匹配。对已发表的ITS序列进行分析预测，贝氏节皮菌和梵氏节皮菌有不同的DdeI限制性酶切位点，而兔分离株所有由DdeI产生的限制性片段长度多态性与梵氏节皮菌的相同。所提出的方法可用于快速检测皮肤癣菌并初步鉴定须癣毛癣菌。
