Smith Darci R, Aguilar Patricia V, Coffey Lark L, Gromowski Gregory D, Wang Eryu, Weaver Scott C
Department of Pathology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-0609, USA.
Emerg Infect Dis. 2006 Aug;12(8):1190-6. doi: 10.3201/eid1708.050841.
Quantifying the dose of an arbovirus transmitted by mosquitoes is essential for designing pathogenesis studies simulating natural infection of vertebrates. Titration of saliva collected in vitro from infected mosquitoes may not accurately estimate titers transmitted during blood feeding, and infection by needle injection may affect vertebrate pathogenesis. We compared the amount of Venezuelan equine encephalitis virus collected from the saliva of Aedes taeniorhynchus to the amount injected into a mouse during blood feeding. Less virus was transmitted by mosquitoes in vivo (geometric mean 11 PFU) than was found for comparable times of salivation in vitro (mean saliva titer 74 PFU). We also observed slightly lower early and late viremia titers in mice that were needle injected with 8 PFU, which represents the low end of the in vivo transmission range. No differences in survival were detected, regardless of the dose or infection route.
量化蚊子传播虫媒病毒的剂量对于设计模拟脊椎动物自然感染的发病机制研究至关重要。体外收集的受感染蚊子唾液的滴定可能无法准确估计吸血过程中传播的滴度，而通过针头注射感染可能会影响脊椎动物的发病机制。我们比较了从黄头伊蚊唾液中收集的委内瑞拉马脑炎病毒量与吸血过程中注入小鼠体内的病毒量。蚊子在体内传播的病毒（几何平均数为11 PFU）比体外可比唾液分泌时间所发现的病毒量少（唾液平均滴度为74 PFU）。我们还观察到，用8 PFU进行针头注射的小鼠，其早期和晚期病毒血症滴度略低，8 PFU代表体内传播范围的下限。无论剂量或感染途径如何，均未检测到存活率的差异。
