Eiteman Mark A, Altman Elliot
Center for Molecular BioEngineering, University of Georgia, Athens, GA 30602, USA.
Trends Biotechnol. 2006 Nov;24(11):530-6. doi: 10.1016/j.tibtech.2006.09.001. Epub 2006 Sep 12.
Escherichia coli is the organism of choice for the expression of a wide variety of recombinant proteins for therapeutic, diagnostic and industrial applications. E. coli generates acetic acid (acetate) as an undesirable by-product that has several negative effects on protein production. Various strategies have been developed to limit acetate accumulation or reduce its negative effects to increase the productivity of recombinant proteins. This article reviews recent strategies for reducing or eliminating acetate, including approaches that optimize the protein production process as well as those that involve modifying the host organism itself.
大肠杆菌是用于表达多种重组蛋白以用于治疗、诊断和工业应用的首选生物体。大肠杆菌会产生乙酸（醋酸盐）作为一种不良副产物，其对蛋白质生产有多种负面影响。已开发出各种策略来限制乙酸积累或降低其负面影响，以提高重组蛋白的产量。本文综述了近期减少或消除乙酸的策略，包括优化蛋白质生产过程的方法以及涉及改造宿主生物体本身的方法。
