Chen M-J, La T, Zhao P, Tam J S, Rappaport R, Cheng S-M
Department of Applied Immunology and Microbiology, Wyeth Vaccines Research, 401 N. Middletown Road, Building 180/256-16, Pearl River, NY 10965, USA.
Virus Res. 2006 Dec;122(1-2):200-5. doi: 10.1016/j.virusres.2006.07.010. Epub 2006 Sep 12.
Genetic analyses were performed on 228 influenza A(H1) viruses derived from clinical subjects participating in an experimental vaccine trial conducted in 20 countries on four continents between 2001 and 2003. HA1 phylogenetic analysis of these viruses showed multiple clades circulated around the world with regional prevalence patterns. Sixty-five of the A(H1) viruses were identified as A(H1N2), 40 of which were isolated from South Africa. The A(H1) sequences of these viruses cluster with published H1N2 viruses phylogenetically and share with them diagnostic signature V169A and A193T changes. The results also showed for the first time that H1N2 viruses were prominent in South Africa during the 2001-2002 influenza season, accounting for over 90% of the A(H1) cases in our study, and infecting both children (29/31) and the elderly (11/13). Phylogenetic analysis of the 65 H1N2 viruses we identified, in conjunction with the 56 recent H1N2 viruses currently available in the database, provided a comprehensive view of the circulation and evolution of distinct clades of H1N2 viruses in a temporal manner between early 2001 and mid-2003, shortly after the appearance of these recent reassortant viruses in or near year 2000.
对228株甲型（H1）流感病毒进行了基因分析，这些病毒来自于2001年至2003年期间在四大洲20个国家开展的一项实验性疫苗试验的临床受试者。对这些病毒的HA1系统发育分析表明，多个进化枝在全球传播，并呈现出区域流行模式。其中65株甲型（H1）病毒被鉴定为甲型（H1N2），其中40株从南非分离得到。这些病毒的甲型（H1）序列在系统发育上与已发表的H1N2病毒聚类，并与它们共享诊断性特征V169A和A193T变化。结果还首次表明，H1N2病毒在2001 - 2002年流感季节在南非较为突出，在我们的研究中占甲型（H1）病例的90%以上，并且感染了儿童（29/31）和老年人（11/13）。对我们鉴定的65株H1N2病毒进行系统发育分析，并结合数据库中现有的56株近期H1N2病毒，以时间顺序全面呈现了2001年初至2003年年中不同进化枝的H1N2病毒在这些近期重配病毒于2000年前后出现后不久的传播和进化情况。
