Giannopoulou C, Di Felice R, Andersen E, Cimasoni G
Division of Physiopathology and Periodontology, School of Dentistry, Medical Faculty, University of Geneva, Switzerland.
Arch Oral Biol. 1990;35(1):13-6. doi: 10.1016/0003-9969(90)90107-l.
Albumin and alpha 2-macroglobulin were analysed in gingival fluid collected by filter paper strips from 6 volunteers before, during and after 10 days of dental plaque accumulation. The two proteins were also determined in samples of venous blood at the beginning of abstention from tooth brushing and 10 days later. A nephelometric technique was used for the analysis of albumin, but alpha 2-macroglobulin was determined by ELISA. The plaque and gingival indices, as well as the flow of gingival fluid, increased significantly during the period of plaque accumulation and then returned to baseline values. The concentrations of the two proteins in blood remained within normal limits. In gingival fluid, however, the concentration of albumin increased about two-fold at the peak of inflammation, while that of alpha 2-macroglobulin increased as much as 6 times. The enhanced passage of alpha 2-macroglobulin into the gingival sulcus in comparison to the entirely plasma-derived albumin was consistent with the concept that the greater excretion of globulin during inflammation must be due to an increased local synthesis.
采用滤纸从6名志愿者身上收集菌斑积聚前、积聚期间和积聚10天后的龈沟液，分析其中的白蛋白和α2-巨球蛋白。在停止刷牙开始时和10天后，还测定了静脉血样本中的这两种蛋白质。采用散射比浊法分析白蛋白，但采用酶联免疫吸附测定法测定α2-巨球蛋白。在菌斑积聚期间，菌斑指数和牙龈指数以及龈沟液流量显著增加，然后恢复到基线值。血液中这两种蛋白质的浓度保持在正常范围内。然而，在龈沟液中，炎症高峰期白蛋白浓度增加约两倍，而α2-巨球蛋白浓度增加多达6倍。与完全来源于血浆的白蛋白相比，α2-巨球蛋白进入龈沟的量增加，这与炎症期间球蛋白排泄增加必定是由于局部合成增加的观点一致。
