Etensel Barlas, Ozkisacik Sezen, Ozkara Esra, Karul Aslihan, Oztan Onur, Yazici Mesut, Gürsoy Harun
Department of Pediatric Surgery, Adnan Menderes University, Aydin, Turkey.
Pediatr Surg Int. 2007 Feb;23(2):177-81. doi: 10.1007/s00383-006-1781-x. Epub 2006 Sep 16.
Prevention of tissue damage after testicular torsion caused by I/R injury is still a clinical and experimental problem. There are many experimental studies made with several chemicals in the literature for decreasing the effect of reactive oxygen species after ischemia and reperfusion. Dexpanthenol (Dxp) is the biologically active alcohol of pantothenic acid. Pantothenic acid increases the content of reduced glutathione, Coenzyme A and ATP in cell. We studied the effect of Dxp on lipid peroxidation and testicular damage. Forty adult rats were separated randomly into five groups: group Sh, Sham-operation; group TD, torsion-detorsion; group NS, torsion-normal saline-detorsion; group D, torsion-Dxp 250 mg/kg detorsion; group D2, torsion-Dxp 500 mg/kg detorsion group. Serum MDA levels were taken before detorsion, after torsion at the first and fifth minute and at the first hour. Tissue sample was taken at the first hour. The alterations of I/R injury on testis were histological graded. Serum MDA levels were significantly lower in group D2 compared to all groups. The histopathology score of group D2 was significantly lower than groups TD, NS and D. Histopathological score and serum MDA levels are strikingly compatible. Dxp attenuated lipid peroxidation and tissue damage at I/R injury. This effect depends on its antioxidant effect with increasingly reduced glutathione, Coenzyme A and ATP. The effect of Dxp on I/R injury has been shown for the first time in the experimental testicular torsion.
预防睾丸扭转缺血/再灌注（I/R）损伤后组织损伤仍是一个临床和实验难题。文献中有许多使用多种化学物质进行的实验研究，目的是减轻缺血再灌注后活性氧的影响。右泛醇（Dxp）是泛酸的生物活性醇。泛酸可增加细胞内还原型谷胱甘肽、辅酶A和三磷酸腺苷的含量。我们研究了Dxp对脂质过氧化和睾丸损伤的影响。将40只成年大鼠随机分为五组：Sh组，假手术组；TD组，扭转-松解组；NS组，扭转-生理盐水-松解组；D组，扭转-Dxp 250mg/kg-松解组；D2组，扭转-Dxp 500mg/kg-松解组。在松解前、扭转后第1分钟和第5分钟以及第1小时采集血清丙二醛（MDA）水平。在第1小时采集组织样本。对睾丸I/R损伤的改变进行组织学分级。与所有组相比，D2组血清MDA水平显著降低。D2组的组织病理学评分显著低于TD组、NS组和D组。组织病理学评分与血清MDA水平显著相关。Dxp减轻了I/R损伤时的脂质过氧化和组织损伤。这种作用取决于其抗氧化作用，可使还原型谷胱甘肽、辅酶A和三磷酸腺苷含量增加。Dxp对I/R损伤的作用首次在实验性睾丸扭转中得到证实。
