Balmer Yves, Vensel William H, Hurkman William J, Buchanan Bob B
Department of Plant and Microbial Biology, University of California, Berkeley, 94720, USA.
Antioxid Redox Signal. 2006 Sep-Oct;8(9-10):1829-34. doi: 10.1089/ars.2006.8.1829.
In recent years, impressive progress has been made in the identification of thioredoxin-linked proteins. However, due to technical difficulties inherent in working with hydrophobic proteins, identifications so far have been restricted to proteins in the soluble fraction. Thus, our knowledge of redox regulated membrane proteins is quite limited. To gain information in this area, the authors have applied an adaptation of the approach based on the fluorescent thiol probe monobromobimane (mBBr) to identify redox-linked proteins of chloroplast thylakoids. By application of this procedure, 14 potential membrane-bound thioredoxin target proteins were identified, including seven new candidates functional in processes associated with photosynthetic electron flow, ATP synthesis, and Photosystem II/Photosystem I state transitions.
近年来，在硫氧还蛋白连接蛋白的鉴定方面取得了令人瞩目的进展。然而，由于处理疏水蛋白存在固有的技术难题，迄今为止的鉴定仅限于可溶性部分的蛋白质。因此，我们对氧化还原调节的膜蛋白的了解相当有限。为了获取该领域的信息，作者采用了一种基于荧光硫醇探针单溴代双马来酰亚胺（mBBr）的方法的改进方法，以鉴定叶绿体类囊体的氧化还原连接蛋白。通过应用该程序，鉴定出了14种潜在的膜结合硫氧还蛋白靶蛋白，包括7种在与光合电子流、ATP合成以及光系统II/光系统I状态转换相关过程中发挥作用的新候选蛋白。
