Singla Kartesh, Sandhu Simarpreet Virk, Pal Rana A G K, Bansal Himanta, Bhullar Ramanpreet Kaur, Kaur Preetinder
Department of Oral Pathology & Microbiology, Sri Sukhmani Dental College & Hospital, Dera Bassi, Punjab, India.
Department of Oral Pathology & Microbiology, Genesis Institute of Dental Sciences & Research, Ferozepur, Punjab, India.
Int J Health Sci (Qassim). 2017 Apr-Jun;11(2):28-34.
Tissue processing for years is carried out by the conventional method, which is a time-consuming technique resulting in 1-day delay in diagnosis. However, in this area of modernization and managed care, rapid diagnosis is increasingly desirable to fulfill the needs of clinicians. The objective of the present study was to compare and determine the positive impact on turnaround times of different tissue processing methods by comparing the color intensity, cytoplasmic details, and nuclear details of the tissues processed by three methods.
A total of sixty biopsied tissues were grossed and cut into three equal parts. One part was processed by conventional method, second by rapid manual, and third by microwave-assisted method. The slides obtained after processing were circulated among four observers for evaluation. Sections processed by the three techniques were subjected to statistical analysis by Kruskal-Wallis test. Cronbach's alpha reliability test was applied to assess the reliability among observers. One-way analysis of variance (ANOVA) was used for comparing mean shrinkage before and after processing.
All observers were assumed to be reliable as the Cronbach's reliability test was statistically significant. The results were statistically non-significant as observed by Kruskal-Wallis test. One-way ANOVA revealed a significant value on comparison of the tissue shrinkage processed by the three techniques. The histological evaluation of the tissues revealed that the nuclear-cytoplasmic contrast was good in tissues processed by microwave, followed by conventional and rapid manual processing techniques. The color intensity of the tissues processed by microwave was crisper, and there was a good contrast between the hematoxylin and eosin-stained areas as compared to manual methods.
The overall quality of tissues from all the three methods was similar. It was not feasible to distinguish between the three techniques by observing the tissue sections. Microwave-assisted tissue processing has reduced the time from sample reception to diagnosis, thus enabling the same-day processing and diagnosis.
多年来一直采用传统方法进行组织处理，这是一种耗时的技术，会导致诊断延迟1天。然而，在这个现代化和管理式医疗的领域，为满足临床医生的需求，快速诊断越来越受到青睐。本研究的目的是通过比较三种方法处理的组织的颜色强度、细胞质细节和细胞核细节，来比较并确定不同组织处理方法对周转时间的积极影响。
总共60个活检组织进行大体检查并切成三等份。一份采用传统方法处理，第二份采用快速手工方法处理，第三份采用微波辅助方法处理。处理后得到的玻片在四名观察者之间传阅进行评估。对三种技术处理的切片进行Kruskal-Wallis检验的统计分析。应用Cronbach's α可靠性检验来评估观察者之间的可靠性。采用单因素方差分析（ANOVA）比较处理前后的平均收缩率。
由于Cronbach's可靠性检验具有统计学意义，所有观察者都被认为是可靠的。Kruskal-Wallis检验观察到结果无统计学意义。单因素方差分析显示，比较三种技术处理的组织收缩率时有显著差异。对组织的组织学评估显示，微波处理的组织中核质对比度良好，其次是传统和快速手工处理技术。与手工方法相比，微波处理的组织颜色强度更清晰，苏木精和伊红染色区域之间有良好的对比度。
三种方法处理的组织的整体质量相似。通过观察组织切片来区分这三种技术是不可行的。微波辅助组织处理减少了从样本接收至诊断的时间，从而实现了当天处理和诊断。
