Maneekarn Niwat, Khamrin Pattara, Chan-it Wisoot, Peerakome Supatra, Sukchai Sujin, Pringprao Kidsadagon, Ushijima Hiroshi
Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
J Clin Microbiol. 2006 Nov;44(11):4113-9. doi: 10.1128/JCM.00954-06. Epub 2006 Sep 20.
Among 175 fecal specimens collected from diarrheic piglets during a surveillance of porcine rotavirus (PoRV) strains in Chiang Mai, Thailand, 39 (22.3%) were positive for group A rotaviruses. Of these, 33.3% (13 of 39) belonged to G3P[19], which was a rare P genotype seldom reported. Interestingly, their VP4 nucleotide sequences were most closely related to human P[19] strains (Mc323 and Mc345) isolated in 1989 from the same geographical area where these PoRV strains were isolated. These P[19] PoRV strains were also closely related to another human P[19] strain (RMC321), isolated from India in 1990. The VP4 sequence identities with human P[19] were 95.4% to 97.4%, while those to a porcine P[19] strain (4F) were only 87.6 to 89.1%. Phylogenetic analysis of the VP4 gene revealed that PoRV P[19] strains clustered with human P[19] strains in a monophyletic branch separated from strain 4F. Analysis of the VP7 gene confirmed that these strains belonged to the G3 genotype and shared 97.7% to 98.3% nucleotide identities with other G3 PoRV strains circulating in the regions. This close genetic relationship was also reflected in the phylogenetic analysis of their VP7 genes. Altogether, the findings provided peculiar evidence that supported the porcine origin of VP4 genes of Mc323 and Mc345 human rotaviruses.
在泰国清迈对猪轮状病毒（PoRV）毒株进行监测期间，从腹泻仔猪采集的175份粪便样本中，39份（22.3%）A组轮状病毒呈阳性。其中，33.3%（39份中的13份）属于G3P[19]，这是一种很少报道的罕见P基因型。有趣的是，它们的VP4核苷酸序列与1989年从分离出这些PoRV毒株的同一地理区域分离的人P[19]毒株（Mc323和Mc345）关系最为密切。这些P[19] PoRV毒株也与1990年从印度分离的另一人P[19]毒株（RMC321）密切相关。与人类P[19]的VP4序列同一性为95.4%至97.4%，而与猪P[19]毒株（4F）的序列同一性仅为87.6%至89.1%。VP4基因的系统发育分析表明，PoRV P[19]毒株与人类P[19]毒株聚集在一个与4F毒株分离的单系分支中。VP7基因分析证实这些毒株属于G3基因型，与该地区流行的其他G3 PoRV毒株的核苷酸同一性为97.7%至98.3%。这种密切的遗传关系在它们VP7基因的系统发育分析中也得到了体现。总之，这些发现提供了独特的证据，支持了Mc323和Mc345人轮状病毒VP4基因的猪源。
