Uhl W, Büchler M, Nevalainen T J, Deller A, Beger H G
Department of Surgery, University of Ulm, West Germany.
J Trauma. 1990 Oct;30(10):1285-90. doi: 10.1097/00005373-199010000-00015.
Catalytic phospholipase A2 activity (CA-PLA2) and the concentration of immunoreactive pancreatic PLA2 (IR-PLA2) were measured in serum samples from 12 patients with multiple injuries (median Injury Severity Score: 41). CA-PLA2 was increased in all patients and positive correlations were found between the extent of the increase of CA-PLA2, mortality, and impairment of pulmonary function. IR-PLA2 values were slightly increased in the serum of nine patients with multiple injuries. Of these nine patients, eight had an additional blunt abdominal trauma. On the other hand, no relationship was found between IR-PLA2 and CA-PLA2 values. This finding was confirmed by immunoadsorption experiments with an antiserum to human pancreatic PLA2, which demonstrated that the increased serum levels of IR-PLA2 were not responsible for the increased CA-PLA2 values. The results suggest the existence of at least two immunologically different phospholipase A2 enzymes in sera of patients with multiple injuries.
对12例多发伤患者（损伤严重度评分中位数：41）的血清样本测定了催化型磷脂酶A2活性（CA-PLA2）和免疫反应性胰腺磷脂酶A2（IR-PLA2）的浓度。所有患者的CA-PLA2均升高，且发现CA-PLA2升高程度、死亡率和肺功能损害之间存在正相关。9例多发伤患者血清中的IR-PLA2值略有升高。在这9例患者中，8例还伴有腹部钝性创伤。另一方面，未发现IR-PLA2与CA-PLA2值之间存在关联。用抗人胰腺PLA2抗血清进行的免疫吸附实验证实了这一发现，该实验表明IR-PLA2血清水平升高并非CA-PLA2值升高的原因。结果提示多发伤患者血清中至少存在两种免疫上不同的磷脂酶A2酶。
