Gaĭdamovich S Ia, Loktev V B, Lavrova N A, Maksiutov A Z, Mel'nikova E E, Pereboev A V, Protopopov E V, Razumov I A, Sveshnikova N A, Khusainova A D
Vopr Virusol. 1990 May-Jun;35(3):221-5.
Employment of radioimmunoassay led to the demonstration of serological crossing between tick-borne encephalitis (TBE) virus and Venezuelan equine encephalomyelitis (VEE) virus. Using hybridoma technology, three hybridomas were produced secreting monoclonal antibodies (MAb) cross-reacting with these two viruses. With MAb, the epitope of binding of these antibodies was shown to be located on protein E of TBE virus and protein E1 of VEE virus. Despite the low percentage (14%) of homology of amino acid sequences of these proteins, 12 areas with homology from 24% to 63% were demonstrated. Considering conservative replacements, homology of these areas was 53%-75%. The assumed existence of some of these areas in alpha-helical conformation may explain the observed immunological crossing.
放射免疫测定法的应用证实了蜱传脑炎（TBE）病毒和委内瑞拉马脑炎（VEE）病毒之间存在血清学交叉反应。利用杂交瘤技术，制备了三种分泌与这两种病毒发生交叉反应的单克隆抗体（MAb）的杂交瘤。利用单克隆抗体表明，这些抗体的结合表位位于TBE病毒的E蛋白和VEE病毒的E1蛋白上。尽管这些蛋白质的氨基酸序列同源性较低（14%），但仍显示出12个同源性在24%至63%之间的区域。考虑到保守替换，这些区域的同源性为53%-75%。这些区域中某些区域以α螺旋构象存在的假设可能解释了所观察到的免疫交叉反应。
