Tsai C H, Williams M V, Glaser R
Department of Medical Microbiology, Ohio State University Medical Center, Columbus 43210.
Proc Natl Acad Sci U S A. 1990 Oct;87(20):7963-7. doi: 10.1073/pnas.87.20.7963.
A monoclonal antibody (mAb) designated 55H3 was produced by using chemically induced Epstein-Barr virus genome-positive B95-8 cells. mAb 55H3, which reacted with an 85- to 80-kDa polypeptide, neutralized Epstein-Barr virus-encoded DNA polymerase activity in crude extracts of chemically induced M-ABA, HR-1, and B95-8 cells, as well as the partially purified Epstein-Barr virus DNA polymerase in a dose-dependent manner. The mAb also neutralized the virus-encoded DNA polymerase activity from cells infected with human cytomegalovirus, human herpesvirus 6, and the purified bacteriophage T4 DNA polymerases. However, mAb 55H3 did not neutralize the DNA polymerase activities encoded for by herpes simplex virus types 1 and 2, the reverse transcriptase of avian myeloblastosis virus, or Escherichia coli DNA polymerase 1 (Klenow fragment). These results suggest that mAb 55H3 recognizes an epitope common to some herpesviruses and T4 DNA polymerases and further supports the hypothesis that these organisms are evolutionarily related.
通过使用化学诱导的爱泼斯坦-巴尔病毒基因组阳性的B95-8细胞产生了一种名为55H3的单克隆抗体（mAb）。与85至80 kDa多肽发生反应的mAb 55H3，以剂量依赖的方式中和了化学诱导的M-ABA、HR-1和B95-8细胞粗提物中爱泼斯坦-巴尔病毒编码的DNA聚合酶活性，以及部分纯化的爱泼斯坦-巴尔病毒DNA聚合酶的活性。该单克隆抗体还中和了来自感染人巨细胞病毒、人疱疹病毒6的细胞以及纯化的噬菌体T4 DNA聚合酶的病毒编码的DNA聚合酶活性。然而，mAb 55H3并未中和1型和2型单纯疱疹病毒编码的DNA聚合酶活性、禽成髓细胞瘤病毒的逆转录酶或大肠杆菌DNA聚合酶1（克列诺片段）的活性。这些结果表明，mAb 55H3识别某些疱疹病毒和T4 DNA聚合酶共有的一个表位，并进一步支持了这些生物体在进化上相关的假说。
