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对洋葱根尖进行测试的五种食品防腐剂的遗传毒性

Genotoxicity of five food preservatives tested on root tips of Allium cepa L.

作者信息

Türkoğlu Sifa

机构信息

Cumhuriyet University, Faculty of Arts and Science, Department of Biology, Sivas, Turkey.

出版信息

Mutat Res. 2007 Jan 10;626(1-2):4-14. doi: 10.1016/j.mrgentox.2006.07.006. Epub 2006 Sep 26.

DOI:10.1016/j.mrgentox.2006.07.006
PMID:17005441
Abstract

The effects of the food preservatives sodium benzoate (SB), boric acid (BA), citric acid (CA), potassium citrate (PC) and sodium citrate (SC) have been studied on root tips of Allium cepa L. Roots of A. cepa were treated with a series of concentrations, ranging from 20 to 100 ppm for 5, 10 and 20 h. The results indicate that these food preservatives reduced mitotic division in A. cepa compared with the respective control. Mitotic index values were generally decreased with increasing concentrations and longer treatment times. Additionally, variations in the percentage of mitotic stages were observed. The total percentage of aberrations generally increased with increasing concentrations of these chemicals and the longer period of treatment. Different abnormal mitotic figures were observed in all mitotic phases. Among these abnormalities were anaphase bridges, C-mitosis, micronuclei, lagging, stickiness, breaks and unequal distribution.

摘要

已对食品防腐剂苯甲酸钠(SB)、硼酸(BA)、柠檬酸(CA)、柠檬酸钾(PC)和柠檬酸钠(SC)对洋葱根尖的影响进行了研究。用一系列浓度(20至100 ppm)处理洋葱根5、10和20小时。结果表明,与各自的对照相比,这些食品防腐剂减少了洋葱的有丝分裂。有丝分裂指数值通常随着浓度增加和处理时间延长而降低。此外,观察到有丝分裂阶段百分比的变化。畸变的总百分比通常随着这些化学物质浓度的增加和处理时间的延长而增加。在所有有丝分裂阶段均观察到不同的异常有丝分裂图像。这些异常包括后期桥、C-有丝分裂、微核、滞后、粘连、断裂和分布不均。

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