乳酸杆菌、双歧杆菌和嗜酸乳杆菌能在外周血单核细胞中诱导促炎和抗炎细胞因子。
Lactobacilli, bifidobacteria and E. coli nissle induce pro- and anti-inflammatory cytokines in peripheral blood mononuclear cells.
作者信息
Helwig Ulf, Lammers Karen M, Rizzello Fernando, Brigidi Patricia, Rohleder Verena, Caramelli Elisabetta, Gionchetti Paolo, Schrezenmeir Juergen, Foelsch Ulrich R, Schreiber Stefan, Campieri Massimo
机构信息
University Hospital Schleswig-Holstein, Campus Kiel, 1st Medical Department, 24105 Kiel, Germany.
出版信息
World J Gastroenterol. 2006 Oct 7;12(37):5978-86. doi: 10.3748/wjg.v12.i37.5978.
AIM
To investigate whether the stimulation of peripheral blood mononuclear cells (PBMNC) with the cell debris and cell extraction of different probiotic strains is similar or species specific.
METHODS
Three strains of bifidobacteria, 4 strains of lactobacilli, and E. coli nissle were sonicated and centrifuged in order to divide them into cell extract and cell debris. PBMNC were separated by density gradient and incubated for 36 h with either the cell debris or the cell extract of single strains of probiotic bacteria in doses from 10(2) to 10(8) CFU/mL. Cell supernatants were taken and interleukin (IL)-10, IL-1beta, and tumor necrosis factor (TNF)-alpha were determined by ELISA.
RESULTS
Depending on the species super-family, the strains had different stimulation patterns. Except for both L. casei strains, the cell extract of bifidobacteria and lactobacilli had less stimulating capacity than cell debris, whereas the cell extract of E. coli nissle had similar stimulating properties to that of the cell debris of the strain and significantly more stimulating capacity than that of bifidobacteria and lactobacilli. The cell debris of bifidobacteria stimulated more cytokine release than the cell debris of lactobacilli. The cell debris of lactobacilli did not have a stimulating capacity when lower concentrations were used. Neither cell extraction nor cell debris had an inhibitory effect on the production of the tested cytokines by stimulated PBMNC.
CONCLUSION
The incubation of probiotic strains, which have been used in clinical trials for inflammatory diseases, with immunocompetent cells leads to different species specific reactions. High IL-10 response to cell debris of bifidobacteria and E. coli nissle can be found. This corresponds to positive effects of bifidobacteria and E. coli nissle in clinical trials for inflammatory bowel disease compared to negative outcomes obtained with lactobacilli.
目的
研究用不同益生菌菌株的细胞碎片和细胞提取物刺激外周血单个核细胞（PBMNC）是具有相似性还是具有种属特异性。
方法
对三株双歧杆菌、四株乳酸杆菌和嗜酸乳杆菌进行超声处理和离心，以将它们分为细胞提取物和细胞碎片。通过密度梯度分离PBMNC，并将其与剂量为10²至10⁸CFU/mL的单一益生菌菌株的细胞碎片或细胞提取物孵育36小时。收集细胞上清液，通过酶联免疫吸附测定法（ELISA）测定白细胞介素（IL）-10、IL-1β和肿瘤坏死因子（TNF）-α。
结果
根据种属超家族的不同，各菌株具有不同的刺激模式。除两株干酪乳杆菌外，双歧杆菌和乳酸杆菌的细胞提取物的刺激能力低于细胞碎片，而嗜酸乳杆菌的细胞提取物与该菌株的细胞碎片具有相似的刺激特性，且刺激能力明显高于双歧杆菌和乳酸杆菌。双歧杆菌的细胞碎片比乳酸杆菌的细胞碎片刺激更多的细胞因子释放。当使用较低浓度时，乳酸杆菌的细胞碎片没有刺激能力。细胞提取物和细胞碎片均未对受刺激的PBMNC产生的受试细胞因子产生抑制作用。
结论
在临床试验中用于治疗炎症性疾病的益生菌菌株与免疫活性细胞孵育会导致不同的种属特异性反应。可以发现双歧杆菌和嗜酸乳杆菌的细胞碎片能引起较高的IL-10反应。这与双歧杆菌和嗜酸乳杆菌在炎症性肠病临床试验中的积极效果相符，而乳酸杆菌的试验结果为阴性。
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