Gálvez A, Valdivia E, Martínez-Bueno M, Maqueda M
Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, Spain.
J Appl Bacteriol. 1990 Sep;69(3):406-13. doi: 10.1111/j.1365-2672.1990.tb01531.x.
In addition to its bactericidal mode of action, the peptide antibiotic AS-48 exhibits a bacteriolytic effect on Enterococcus faecalis S-47 that is associated with autolysin activation. Bacteriolysis induced by the antibiotic can be modulated by addition of EDTA, divalent cations and autolysin activators (trypsin) or inhibitors (cardiolipin), suggesting that topologic regulation of the autolysins is involved in the process. In addition, inhibitors of protein and RNA synthesis interfere markedly with bacteriolysis, as do ionophores and the ATPase inhibitor DCCD, suggesting the participation of an internal messenger in autolysin activation in the presence of AS-48.
除了其杀菌作用方式外，肽抗生素AS-48对粪肠球菌S-47具有溶菌作用，这与自溶素激活有关。抗生素诱导的溶菌作用可通过添加EDTA、二价阳离子和自溶素激活剂（胰蛋白酶）或抑制剂（心磷脂）来调节，这表明自溶素的拓扑调节参与了该过程。此外，蛋白质和RNA合成抑制剂以及离子载体和ATP酶抑制剂DCCD对溶菌作用有显著干扰，这表明在AS-48存在的情况下，一种内部信使参与了自溶素的激活。
