Seo Boram, Jeon Kyungchan, Baek Inwoo, Lee Yung Mi, Baek Kiwoon, Ko GwangPyo
1Department of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Seoul, Republic of Korea.
2School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
Int J Syst Evol Microbiol. 2019 Jul;69(7):2120-2128. doi: 10.1099/ijsem.0.003443. Epub 2019 May 17.
Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains, SNUG30099 and SNUG30370, were isolated from faecal samples of healthy Korean subjects. The strains formed circular ivory-coloured colonies on Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar and cells were approximately 3.5-4.5×0.3-0.4 µm in size. Taxonomic analyses based on 16S rRNA gene sequences distinguished the strains from other species within the family Erysipelotrichaceae. The closest relative of strains SNUG30099 and SNUG30370 was Longibaculum muris (92.9 % and 93.6 % similarity, respectively), followed by Clostridium saccharogumia (92.3 % and 92.2 %). Phylogenetic inference also divided the strains into a unique branch that differed from other related strains that belong to the family Erysipelotrichaceae. DNA G+C contents based on the whole genome sequences of strains SNUG30099 and SNUG30370 were 29.2 and 30.2 mol%, respectively. Both novel strains possessed meso-diaminopimelic acid as the peptidoglycan, and phosphatidylethanolamine was observed as one of the major polar lipids. The major cellular fatty acid composition was different from those of other related taxa. In addition, the profile of biochemical activities advocated that the strains have distinct characteristics in comparison to other strains. Taken together, a novel genus, named Faecalibacillus gen. nov., is proposed, which includes the type species Faecalibacillus intestinalis sp. nov. for strain SNUG30099 and Faecalibacillus faecis sp. nov. for strain SNUG30370. The type strains of these novel species are SNUG30099 (=KCTC 15631=JCM 32256) and SNUG30370 (=KCTC 15632=JCM 32257).
从健康韩国受试者的粪便样本中分离出两株革兰氏染色阳性、长杆状、专性厌氧且不形成芽孢的菌株，分别为SNUG30099和SNUG30370。这些菌株在添加了0.5% 迪福酵母提取物（YBHI）琼脂的脑心浸液培养基上形成圆形象牙色菌落，细胞大小约为3.5 - 4.5×0.3 - 0.4 µm。基于16S rRNA基因序列的分类分析将这些菌株与丹毒丝菌科内的其他物种区分开来。菌株SNUG30099和SNUG30370的最相近亲缘种是鼠长杆菌（相似性分别为92.9%和93.6%），其次是蔗糖梭菌（92.3%和92.2%）。系统发育推断也将这些菌株分为一个独特的分支，与丹毒丝菌科的其他相关菌株不同。基于菌株SNUG30099和SNUG30370全基因组序列的DNA G + C含量分别为29.2 mol%和30.2 mol%。这两株新菌株均以中 - 二氨基庚二酸作为肽聚糖，磷脂酰乙醇胺被观察为主要极性脂质之一。主要细胞脂肪酸组成与其他相关分类单元不同。此外，生化活性谱表明这些菌株与其他菌株相比具有独特特征。综上所述，提出了一个新属，命名为粪便芽孢杆菌属（Faecalibacillus gen. nov.），其中包括模式种肠道粪便芽孢杆菌（Faecalibacillus intestinalis sp. nov.）（菌株SNUG30099）和粪便粪便芽孢杆菌（Faecalibacillus faecis sp. nov.）（菌株SNUG30370）。这些新物种的模式菌株分别为SNUG30099（=KCTC 15631 = JCM 32256）和SNUG30370（=KCTC 15632 = JCM 32257）。
