Deng Bo, Wu Jie, Li Xiaohui, Men Xiaoming, Xu Ziwei
Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Curr Microbiol. 2017 Nov;74(11):1306-1315. doi: 10.1007/s00284-017-1318-7. Epub 2017 Jul 31.
In the present study, we sought to determine the effects of Bacillus subtilis (BAS) and Bacillus licheniformis (BAL) in rats after lipopolysaccharide (LPS)-induced acute intestinal inflammation. We also determined whether the B. subtilis metabolic product (BASM) is as effective as the live-cell probiotic. 60 male SD rats were randomly assigned to five groups and administered a diet containing 0.05% B. licheniformis (BAL group), 0.05% B. subtilis (BAS group), 0.5% B. subtilis metabolic product (BASM group), or a basic diet (PC group and NC group) for 40 days. On day 40, BAL, BAS, BASM, and NC groups were injected with 4 mg/kg body weight LPS. 4 h later, all rats were anesthetized and sacrificed. The results showed that the administration of B. licheniformis and B. subtilis improved intestinal function as evidenced by histology, increased enzyme activity, and mucosal thickness. They also increased the number of intraepithelial lymphocytes and decreased mucosal myeloperoxidase activity and plasma TNF-α. In addition, the cecal content of B. subtilis-treated rats had significantly increased microbial diversity, decreased numbers of Firmicutes, and increased numbers of Bacteroidetes as compared to rats fed basic diets. Similar to BAS group, the cecal content of B. licheniformis-treated rats decreased the number of Firmicutes. Administration of B. subtilis metabolic product had similar effects on intestinal function, inflammation response, and microbial diversity as B. subtilis but these effects were attenuated. In conclusion, administration of probiotic strains B. licheniformis or B. subtilis improved intestinal function, ameliorated the inflammation response, and modulated microflora after LPS-induced acute inflammation in rats. Non-living cells also exerted probiotic properties but live cells tended to function better.
在本研究中，我们试图确定枯草芽孢杆菌（BAS）和地衣芽孢杆菌（BAL）对脂多糖（LPS）诱导的大鼠急性肠道炎症的影响。我们还确定了枯草芽孢杆菌代谢产物（BASM）是否与活细胞益生菌一样有效。将60只雄性SD大鼠随机分为五组，分别给予含0.05%地衣芽孢杆菌的饮食（BAL组）、0.05%枯草芽孢杆菌的饮食（BAS组）、0.5%枯草芽孢杆菌代谢产物的饮食（BASM组）或基础饮食（PC组和NC组），持续40天。在第40天，给BAL组、BAS组、BASM组和NC组大鼠注射4mg/kg体重的LPS。4小时后，将所有大鼠麻醉并处死。结果表明，给予地衣芽孢杆菌和枯草芽孢杆菌可改善肠道功能，组织学、酶活性增加和黏膜厚度增加均证明了这一点。它们还增加了上皮内淋巴细胞数量，降低了黏膜髓过氧化物酶活性和血浆TNF-α水平。此外，与喂食基础饮食的大鼠相比，枯草芽孢杆菌处理的大鼠盲肠内容物的微生物多样性显著增加，厚壁菌门数量减少，拟杆菌门数量增加。与BAS组相似，地衣芽孢杆菌处理的大鼠盲肠内容物中厚壁菌门数量减少。给予枯草芽孢杆菌代谢产物对肠道功能、炎症反应和微生物多样性的影响与枯草芽孢杆菌相似，但这些影响有所减弱。总之，给予益生菌菌株地衣芽孢杆菌或枯草芽孢杆菌可改善大鼠LPS诱导的急性炎症后的肠道功能，减轻炎症反应并调节微生物群。非活细胞也具有益生菌特性，但活细胞的功能往往更好。
