Sticherling M, Bornscheuer E, Schröder J M, Christophers E
Department of Dermatology, University of Kiel, F.R.G.
J Invest Dermatol. 1991 Jan;96(1):26-30. doi: 10.1111/1523-1747.ep12514689.
Various cytokines have in the past been detected in human skin. Among these, the neutrophil-activating peptide NAP-1/IL-8 is a potent 8-kD proinflammatory peptide that has been purified from psoriatic scales. Its chemotactic activity on human neutrophils, as well as its presence in psoriatic scales, may relate to a role in this disease. In the present study, the tissue distribution of the peptide was examined immunohistochemically using two monoclonal antibodies (52E8, 46E5) recently produced and characterized in our laboratory. Immunoreactivity was detected in both normal and psoriatic skin, resulting in uniform suprabasal keratinocyte staining in normal skin with 52E8 and of all keratinocytes with 46E5. Immunoreactivity in psoriasis correlated to the inflammatory tissue reaction, varying from uniform absence in highly active psoriasis to focally weak staining in plaque type psoriasis. Cells of the acrosyringium and hair follicles were always positive and were unaffected by the inflammatory activity. Epidermal immunoreactivity detected in this study may be associated with closely related peptides of the IL8 family or with truncated or extended forms of NAP-1/IL-8.
过去在人类皮肤中检测到多种细胞因子。其中，中性粒细胞激活肽NAP-1/IL-8是一种有效的8 kDa促炎肽，已从银屑病鳞屑中纯化出来。它对人类中性粒细胞的趋化活性以及在银屑病鳞屑中的存在，可能与该疾病的发生机制有关。在本研究中，使用我们实验室最近制备并鉴定的两种单克隆抗体（52E8、46E5）通过免疫组织化学方法检测了该肽的组织分布。在正常皮肤和银屑病皮肤中均检测到免疫反应性，52E8抗体使正常皮肤的基底上层角质形成细胞呈现均匀染色，46E5抗体则使所有角质形成细胞染色。银屑病中的免疫反应性与炎症组织反应相关，从高度活跃的银屑病中完全缺失到斑块型银屑病中的局灶性弱阳性染色不等。顶泌汗腺导管和毛囊的细胞始终呈阳性，且不受炎症活动的影响。本研究中检测到的表皮免疫反应性可能与IL8家族密切相关的肽或NAP-1/IL-8的截短或延长形式有关。
