Ji Chao, Lu Yuxiao, Li Juan, Hua Marti Z, Xie Yuxin, Ma Ying, Shi Rui, Zhao Liangjuan, Yang Min, He Xiahong, Zheng Wenjie, Lu Xiaonan
Laboratory for Quality Control and Traceability of Food and Agricultural Products, Tianjin Normal University, Tianjin 300387, China.
Department of Food Science and Agricultural Chemistry, Faculty of Agricultural and Environmental Sciences, McGill University, Sainte-Anne-de-Bellevue, Quebec H9X 3 V9, Canada.
ACS Omega. 2023 Jul 18;8(30):27450-27457. doi: 10.1021/acsomega.3c02962. eCollection 2023 Aug 1.
Dencichine is a nonprotein amino acid, an effective ingredient in with hemostatic and anti-inflammatory effects. There are few studies on the effects of regions and cultivation models on the accumulation of dencichine. In the current study, the content of dencichine in collected from its global cultivation and trading center Yunnan, China, (>640 samples) was determined using an optimized high-performance liquid chromatography method coupled with a diode array detector but without derivatization. The recovery rate of this method was 80-110%, the relative standard deviation was <10%, and the limits of detection and quantification were 0.003% (w/w) and 0.01% (w/w), respectively. The content of dencichine in each part of was as follows: rootlets (39.59%) > main roots (29.91%) > leaves (16.21%) > stems (14.29%). For leaves, in the forest (5.52 ± 2.26 mg/g) was significantly higher than that in the field (3.93 ± 1.72 mg/g) but opposite for main roots. The origins and altitudes made different contributions to the accumulation of dencichine in . This study provides an effective analytical method to determine dencichines in various parts of from different origins and altitudes and supports quality control and product development of .
三七素是一种非蛋白质氨基酸，是三七中具有止血和抗炎作用的有效成分。关于产地和种植模式对三七素积累的影响的研究较少。在本研究中，采用优化的高效液相色谱法结合二极管阵列检测器但无需衍生化，测定了从其全球种植和贸易中心中国云南收集的三七（>640个样品）中三七素的含量。该方法的回收率为80-110%，相对标准偏差<10%，检测限和定量限分别为0.003%（w/w）和0.01%（w/w）。三七各部位的三七素含量如下：须根（39.59%）>主根（29.91%）>叶（16.21%）>茎（14.29%）。对于叶片，林下三七（5.52±2.26mg/g）显著高于田间三七（3.93±1.72mg/g），但主根情况相反。产地和海拔对三七中三七素的积累有不同贡献。本研究提供了一种有效的分析方法来测定不同产地和海拔的三七各部位的三七素，为三七的质量控制和产品开发提供了支持。
