Nielsen K, Sørensen P G, Hynne F, Busse H G
Department of Chemistry and CATS, H.C. Ørsted Institute, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark.
Biophys Chem. 1998 May 5;72(1-2):49-62. doi: 10.1016/s0301-4622(98)00122-7.
We report sustained oscillations in glycolysis conducted in an open system (a continuous-flow, stirred tank reactor; CSTR) with inflow of yeast extract as well as glucose. Depending on the operating conditions, we observe simple or complex periodic oscillations or chaos. We report the response of the system to instantaneous additions of small amounts of several substrates as functions of the amount added and the phase of the addition. We simulate oscillations and perturbations by a kinetic model based on the mechanism of glycolysis in a CSTR. We find that the response to particular perturbations forms an efficient tool for elucidating the mechanism of biochemical oscillations.
我们报告了在开放系统（连续流动搅拌槽式反应器；CSTR）中进行的糖酵解持续振荡，该系统有酵母提取物和葡萄糖流入。根据操作条件，我们观察到简单或复杂的周期性振荡或混沌现象。我们报告了系统对几种底物少量瞬时添加的响应，该响应是添加量和添加阶段的函数。我们通过基于CSTR中糖酵解机制的动力学模型来模拟振荡和扰动。我们发现，对特定扰动的响应构成了阐明生化振荡机制的有效工具。
