Larrondo Luis F, Canessa Paulo, Vicuña Rafael, Stewart Philip, Vanden Wymelenberg Amber, Cullen Dan
Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile and Instituto Milenio de Biología Fundamental y Aplicada, Santiago, Chile.
Mol Genet Genomics. 2007 Jan;277(1):43-55. doi: 10.1007/s00438-006-0167-z. Epub 2006 Oct 11.
We describe the structure, organization, and transcriptional impact of repetitive elements within the lignin-degrading basidiomycete, Phanerochaete chrysosporium. Searches of the P. chrysosporium genome revealed five copies of pce1, a ~1,750-nt non-autonomous, class II element. Alleles encoding a putative glucosyltransferase and a cytochrome P450 harbor pce insertions and produce incomplete transcripts. Class I elements included pcret1, an intact 8.14-kb gypsy-like retrotransposon inserted within a member of the multicopper oxidase gene family. Additionally, we describe a complex insertion of nested transposons within another putative cytochrome P450 gene. The disrupted allele lies within a cluster of >14 genes, all of which encode family 64 cytochrome P450s. Components of the insertion include a disjoint copia-like element, pcret3, the pol domain of a second retroelement, pcret2, and a duplication of an extended ORF of unknown function. As in the case of the pce elements, pcret1 and pcret2/3 insertions are confined to single alleles, transcripts of which are truncated. The corresponding wild-type alleles are apparently unaffected. In aggregate, P. chrysosporium harbors a complex array of repetitive elements, at least five of which directly influence expression of genes within families of structurally related sequences.
我们描述了木质素降解担子菌黄孢原毛平革菌（Phanerochaete chrysosporium）中重复元件的结构、组织及转录影响。对黄孢原毛平革菌基因组的搜索揭示了pce1的五个拷贝，pce1是一个约1750个核苷酸的非自主II类元件。编码假定葡糖基转移酶和细胞色素P450的等位基因含有pce插入序列，并产生不完整的转录本。I类元件包括pcret1，这是一个完整的8.14 kb类gypsy逆转座子，插入到多铜氧化酶基因家族的一个成员中。此外，我们描述了另一个假定细胞色素P450基因内嵌套转座子的复杂插入。被破坏的等位基因位于一个由超过14个基因组成的簇内，所有这些基因都编码64家族细胞色素P450。插入的成分包括一个不连续的类copia元件pcret3、第二个逆转元件pcret2的pol结构域以及一个功能未知的延伸开放阅读框的重复序列。与pce元件的情况一样，pcret1和pcret2/3插入仅限于单个等位基因，其转录本被截断。相应的野生型等位基因显然未受影响。总体而言，黄孢原毛平革菌含有一系列复杂的重复元件，其中至少有五个直接影响结构相关序列家族内基因的表达。
