益生菌菌株的免疫调节作用：采用放射性标记测定法对从虹鳟（Oncorhynchus mykiss）肠道分离的白细胞对杀鲑气单胞菌的吞噬作用进行定量分析。
Immune modulation by probiotic strains: quantification of phagocytosis of Aeromonas salmonicida by leukocytes isolated from gut of rainbow trout (Oncorhynchus mykiss) using a radiolabelling assay.
作者信息
Balcázar José Luis, Vendrell Daniel, de Blas Ignacio, Ruiz-Zarzuela Imanol, Gironés Olivia, Muzquiz José Luis
机构信息
Laboratory of Fish Pathology, University of Zaragoza, Zaragoza 50013, Spain.
出版信息
Comp Immunol Microbiol Infect Dis. 2006 Nov;29(5-6):335-43. doi: 10.1016/j.cimid.2006.09.004. Epub 2006 Oct 10.
A method is described for the rapid and sensitive assay of phagocytosis that utilizes radioactively labelled bacteria. With this method, we observed that phagocytosis of heat-inactivated Aeromonas salmonicida by leukocytes isolated from gut of rainbow trout fed with different viable probiotics (Lactococcus lactis subsp. lactis, Lactobacillus sakei, and Leuconostoc mesenteroides) was significantly higher (P<0.05) after 2 weeks of probiotic-feeding than the control group. However, only phagocytosis of live A. salmonicida by the leukocytes isolated from gut of rainbow trout fed with L. lactis subsp. lactis was significantly higher (P<0.05) than the control group.
描述了一种利用放射性标记细菌对吞噬作用进行快速灵敏检测的方法。通过该方法，我们观察到，在用不同的活益生菌（乳酸乳球菌乳酸亚种、清酒乳杆菌和肠膜明串珠菌）喂养的虹鳟鱼肠道中分离出的白细胞，在喂食益生菌2周后，对热灭活的杀鲑气单胞菌的吞噬作用显著高于（P<0.05）对照组。然而，仅在用乳酸乳球菌乳酸亚种喂养的虹鳟鱼肠道中分离出的白细胞对活的杀鲑气单胞菌的吞噬作用显著高于（P<0.05）对照组。
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