Tsuchida N, Gilden R V, Hatanaka M
J Virol. 1975 Oct;16(4):832-7. doi: 10.1128/JVI.16.4.832-837.1975.
B-34 is the designation of a hamster tumor-derived cell line induced by the Harvey sarcoma virus. This cell line produces virions which contain structural proteins common to edogenous hamster viruses and nucleic acid sequences of hamster, mouse, and rat origin. The sedimentation characteristics of the intracellular virus-specific RNA was determined in sucrose gradients after treatment with dimethylsulfoxide by molecular hybridization using complementary DNA of strict virus specificity. Hamster virus-specific RNA sedimented at 35S (major peak) as is characteristic of productive infection by type C leukemia viruses of other species. Rat virus-specific RNA sedimented at 30S which is characteristic of the sarcoma virus-related genome found in nonproducer cells transformed by Kirsten sarcoma virus. Both Harvey and Kirsten sarcoma viruses contain a related but not necessarily identical 30S rat-specific component which is also found in normal cultured rat cells. Mouse cells producing Harvey sarcoma virus also contain a rat-specific 30S RNA. Mouse virus-derived sequences also sedimented at 30S in B-34 cells and in a similar size range in Harvey virus-infected mouse cells. The possibility that the mouse and rat-derived sequences are present on a single 30S RNA species which would then be related to sarcomagenic potential is one attractive hypothesis suggested by these data.
B - 34是一种由哈维肉瘤病毒诱导产生的仓鼠肿瘤衍生细胞系。该细胞系产生的病毒粒子含有内源性仓鼠病毒共有的结构蛋白以及仓鼠、小鼠和大鼠来源的核酸序列。在用二甲基亚砜处理后，通过使用具有严格病毒特异性的互补DNA进行分子杂交，在蔗糖梯度中测定细胞内病毒特异性RNA的沉降特性。仓鼠病毒特异性RNA沉降在35S（主峰），这是其他物种的C型白血病病毒产生性感染的特征。大鼠病毒特异性RNA沉降在30S，这是在由 Kirsten 肉瘤病毒转化的非产生细胞中发现的与肉瘤病毒相关基因组的特征。哈维和 Kirsten 肉瘤病毒都含有一个相关但不一定相同的30S大鼠特异性成分，在正常培养的大鼠细胞中也能发现。产生哈维肉瘤病毒的小鼠细胞也含有大鼠特异性的30S RNA。小鼠病毒衍生序列在B - 34细胞中也沉降在30S，在感染哈维病毒的小鼠细胞中沉降在类似大小范围内。这些数据提出了一个有吸引力的假设，即小鼠和大鼠衍生序列存在于单个30S RNA物种上，这可能与致癌潜力有关。
